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AB318507

APC Anti-CYP11A1 antibody [EPR24868-86]

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Rabbit Recombinant Monoclonal CYP11A1 antibody - conjugated to APC.

View Alternative Names

CYP11A, CYP11A1, CYPXIA1, Cholesterol desmolase, Cytochrome P450 11A1, Cytochrome P450(scc)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24868-86

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ICC application does not react with Mouse and Rat species. Flow Cyt application does not react with Mouse species.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CYP11A1 also called cytochrome P450scc is an important enzyme that catalyzes the first step in steroid hormone biosynthesis. This enzyme has a molecular weight of approximately 60 kDa. It is primarily located in the mitochondria of steroidogenic tissues such as the adrenal cortex testes and ovaries. CYP11A1 converts cholesterol into pregnenolone a precursor for all steroid hormones. Its gene expression is tightly regulated by trophic hormones which play an important role in modulating steroid production.
Biological function summary

CYP11A1 is a member of the cytochrome P450 superfamily a large group of enzymes known for their role in oxidation and metabolism. It exists in the mitochondrial electron transport chain working closely with proteins such as ferredoxin and ferredoxin reductase. These interactions facilitate the conversion of cholesterol to pregnenolone. Through these biochemical activities CYP11A1 controls the synthesis rate of steroid hormones influencing various physiological functions including metabolism immune response and reproductive system dynamics.

Pathways

CYP11A1 operates within the steroidogenesis pathway which is essential for the synthesis of steroid hormones like cortisol and aldosterone. It directly interacts with StAR protein which transports cholesterol into mitochondria initiating the steroidogenesis process. Moreover CYP11A1 fits into the pregnenolone biosynthesis pathway showing functional connectivity with other cytochrome P450 enzymes like CYP17A1. These interconnected pathways highlight the role of CYP11A1 as a vital catalyst in hormone synthesis affecting both endocrine and metabolic pathways.

CYP11A1 mutations or expression abnormalities have associations with conditions such as congenital adrenal hyperplasia (CAH) and primary adrenal insufficiency. CAH arises from enzyme deficiencies in the steroidogenesis pathway and changes in CYP11A1 activity can exacerbate the disorder by altering cortisol and aldosterone synthesis. Additionally the enzyme’s link with StAR protein suggests a possible connection to lipoid congenital adrenal hyperplasia where defects in cholesterol transport may disrupt steroid production. Understanding these relationships helps in exploring therapeutic targets for steroid-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones (PubMed : 21636783). Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal (PubMed : 21636783). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed : 21636783).
See full target information CYP11A1
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