AB224984

APC Anti-Cytokeratin 5 antibody [EP1601Y]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 5 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5

1 Images

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

Overlay histogram showing A431 cells stained with ab224984 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224984, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

578 PE

PE Anti-Cytokeratin 5 antibody [EP1601Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 5 antibody [EP1601Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 5 antibody [EP1601Y]
Unconjugated

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 5 antibody [EP1601Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1601Y

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

See full target information Keratin, type II cytoskeletal 5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Nature 646:404-412 PubMed40836097

2025

SLC45A4 is a pain gene encoding a neuronal polyamine transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Steven J Middleton,Sigurbjörn Markússon,Mikael Åkerlund,Justin C Deme,Mandy Tseng,Wenqianglong Li,Sana R Zuberi,Gabriel Kuteyi,Peter Sarkies,Georgios Baskozos,Jimena Perez-Sanchez,Adham Farah,Harry L Hébert,Sylvanus Toikumo,Zhanru Yu,Susan Maxwell,Yin Y Dong,Benedikt M Kessler,Henry R Kranzler,John E Linley,Blair H Smith,Susan M Lea,Joanne L Parker,Valeriya Lyssenko,Simon Newstead,David L Bennett

Military Medical Research 9:13 PubMed35351192

2022

Small molecules facilitate single factor-mediated sweat gland cell reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai-Fei Ji,Lai-Xian Zhou,Zhi-Feng Sun,Jiang-Bing Xiang,Shao-Yuan Cui,Yan Li,Hua-Ting Chen,Yi-Qiong Liu,Huan-Huan Gao,Xiao-Bing Fu,Xiao-Yan Sun

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2103079 PubMed34569165

2021

Sweat Gland Organoids Originating from Reprogrammed Epidermal Keratinocytes Functionally Recapitulated Damaged Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Sun,Jiangbing Xiang,Runkai Chen,Zhijun Geng,Lintao Wang,Yiqiong Liu,Shuaifei Ji,Huating Chen,Yan Li,Cuiping Zhang,Peng Liu,Tao Yue,Lei Dong,Xiaobing Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-Cytokeratin 5 antibody [EP1601Y]

Flow Cytometry (Intracellular) - APC Anti-Cytokeratin 5 antibody [EP1601Y] (AB224984)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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