AB234278

APC Anti-DEP1 antibody [MEM-CD148/05]

Name: Anti-DEP1 APC antibody [MEM-CD148/05] (ab234278) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB234278

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(2 Publications)

Mouse Monoclonal DEP1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRJ aa 1-450.

View Alternative Names

CD148, DEP1, PTPRJ, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, HPTP eta, Protein-tyrosine phosphatase receptor type J, DEP-1, R-PTP-J

1 Images

Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (AB234278)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (AB234278)

Flow cytometric analysis of human peripheral blood labeling DEP1 using ab234278 at 10 μl/10⁶ cells. Surface staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-CD148/05

Isotype

IgG2b

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human PTPRJ aa 1-450. The exact immunogen used to generate this antibody is proprietary information.

Q12913

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab234278 is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEP1 also known as protein tyrosine phosphatase receptor type J (PTPRJ) is an important regulator in cell signaling. Its molecular mass is approximately 135-150 kDa. DEP1 is a transmembrane protein expressed in many tissues like epithelial cells endothelial cells and hematopoietic cells. It functions as a protein tyrosine phosphatase meaning it removes phosphate groups from tyrosine residues on target proteins which impacts many cellular processes.

Biological function summary

DEP1 acts as a negative regulator of cell proliferation differentiation and migration. It plays a role in maintaining cellular homeostasis and is often found in complex with other signaling molecules at cell-cell junctions. DEP1 modulates signaling pathways by dephosphorylating receptor tyrosine kinases or their downstream targets. This regulation impacts how cells respond to external stimuli and maintain normal physiological activities.

Pathways

DEP1 is active in both the RAS/MAPK signaling pathway and the PI3K/AKT pathway. It interacts with signaling molecules such as epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor (VEGFR) impacting their signaling abilities. By dephosphorylating these receptors DEP1 modulates the pathways that control cell growth and survival influencing how cells react to growth factors and other signals.

DEP1 connects to several cancers and cardiovascular diseases. It often acts as a tumor suppressor by inhibiting signaling pathways that promote uncontrolled cell growth. In cancer altered DEP1 expression or function can lead to changes in cellular behaviors contributing to tumorigenesis. It relates to proteins like VEGFR in these contexts as their dysregulation can lead to abnormal vascularization in tumors and in cardiovascular disease affecting angiogenesis and blood vessel integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2 (PubMed : 10821867, PubMed : 12062403, PubMed : 12370829, PubMed : 12475979, PubMed : 18348712, PubMed : 19494114, PubMed : 19922411, PubMed : 21262971). Plays a role in cell adhesion, migration, proliferation and differentiation (PubMed : 12370829, PubMed : 14709717, PubMed : 16682945, PubMed : 19836242). Has a role in megakaryocytes and platelet formation (PubMed : 30591527). Involved in vascular development (By similarity). Regulator of macrophage adhesion and spreading (By similarity). Positively affects cell-matrix adhesion (By similarity). Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation (PubMed : 16682945). Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR (PubMed : 21091576). Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation (PubMed : 18936167). Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation (PubMed : 19836242). Enhances the barrier function of epithelial junctions during reassembly (PubMed : 19332538). Negatively regulates T-cell receptor (TCR) signaling (PubMed : 11259588, PubMed : 9531590, PubMed : 9780142). Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling (PubMed : 11259588, PubMed : 12913111).. Isoform 2. Activates angiogenesis and cell migration (PubMed : 28052032). Downregulates the expression of the endothelial adhesion molecules ICAM1 and VCAM1 (PubMed : 28052032).

See full target information PTPRJ

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e36483 PubMed39253182

2024

Navitoclax safety, tolerability, and effect on biomarkers of senescence and neurodegeneration in aged nonhuman primates.

Applications

Unspecified application

Species

Unspecified reactive species

Edward F Greenberg,Martin J Voorbach,Alexandra Smith,David R Reuter,Yuchuan Zhuang,Ji-Quan Wang,Dustin W Wooten,Elizabeth Asque,Min Hu,Carolin Hoft,Ryan Duggan,Matthew Townsend,Karin Orsi,Karen Dalecki,Willi Amberg,Lori Duggan,Heather Knight,Joseph S Spina,Yupeng He,Kennan Marsh,Vivian Zhao,Suzanne Ybarra,Jennifer Mollon,Yuni Fang,Aparna Vasanthakumar,Susan Westmoreland,Mathias Droescher,Sjoerd J Finnema,Hana Florian

Developmental cell 57:2257-2272.e5 PubMed36182685

2022

Pluripotency factors are repurposed to shape the epigenomic landscape of neural crest cells.

Applications

Unspecified application

Species

Unspecified reactive species

Austin S Hovland,Debadrita Bhattacharya,Ana Paula Azambuja,Dimitrius Pramio,Jacqueline Copeland,Megan Rothstein,Marcos Simoes-Costa

View all publications

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Product promise

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APC Anti-DEP1 antibody [MEM-CD148/05]

Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (AB234278)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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