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Mouse Monoclonal DEP1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRJ aa 1-450.

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Images

Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (AB234278), expandable thumbnail

Key facts

Isotype
IgG2b
Host species
Mouse
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

pH: 7.4
Preservative: 0.0975% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human PTPRJ aa 1-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q12913

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
10 µL for 106 Cells
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2 (PubMed:10821867, PubMed:12062403, PubMed:12370829, PubMed:12475979, PubMed:18348712, PubMed:19494114, PubMed:19922411, PubMed:21262971). Plays a role in cell adhesion, migration, proliferation and differentiation (PubMed:12370829, PubMed:14709717, PubMed:16682945, PubMed:19836242). Has a role in megakaryocytes and platelet formation (PubMed:30591527). Involved in vascular development (By similarity). Regulator of macrophage adhesion and spreading (By similarity). Positively affects cell-matrix adhesion (By similarity). Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation (PubMed:16682945). Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR (PubMed:21091576). Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation (PubMed:18936167). Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation (PubMed:19836242). Enhances the barrier function of epithelial junctions during reassembly (PubMed:19332538). Negatively regulates T-cell receptor (TCR) signaling (PubMed:11259588, PubMed:9531590, PubMed:9780142). Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling (PubMed:11259588, PubMed:12913111). Isoform 2. Activates angiogenesis and cell migration (PubMed:28052032). Downregulates the expression of the endothelial adhesion molecules ICAM1 and VCAM1 (PubMed:28052032).

Alternative names

CD148, DEP1, PTPRJ, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, HPTP eta, Protein-tyrosine phosphatase receptor type J, DEP-1, R-PTP-J

Recommended products

Mouse Monoclonal DEP1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRJ aa 1-450.

Key facts

Isotype
IgG2b
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human PTPRJ aa 1-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q12913
Clone number
MEM-CD148/05
Purification technique
Size-exclusion chromatography
Concentration
Loading...
Purification notes

ab234278 is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DEP1 also known as protein tyrosine phosphatase receptor type J (PTPRJ) is an important regulator in cell signaling. Its molecular mass is approximately 135-150 kDa. DEP1 is a transmembrane protein expressed in many tissues like epithelial cells endothelial cells and hematopoietic cells. It functions as a protein tyrosine phosphatase meaning it removes phosphate groups from tyrosine residues on target proteins which impacts many cellular processes.

Biological function summary

DEP1 acts as a negative regulator of cell proliferation differentiation and migration. It plays a role in maintaining cellular homeostasis and is often found in complex with other signaling molecules at cell-cell junctions. DEP1 modulates signaling pathways by dephosphorylating receptor tyrosine kinases or their downstream targets. This regulation impacts how cells respond to external stimuli and maintain normal physiological activities.

Pathways

DEP1 is active in both the RAS/MAPK signaling pathway and the PI3K/AKT pathway. It interacts with signaling molecules such as epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor (VEGFR) impacting their signaling abilities. By dephosphorylating these receptors DEP1 modulates the pathways that control cell growth and survival influencing how cells react to growth factors and other signals.

Associated diseases and disorders

DEP1 connects to several cancers and cardiovascular diseases. It often acts as a tumor suppressor by inhibiting signaling pathways that promote uncontrolled cell growth. In cancer altered DEP1 expression or function can lead to changes in cellular behaviors contributing to tumorigenesis. It relates to proteins like VEGFR in these contexts as their dysregulation can lead to abnormal vascularization in tumors and in cardiovascular disease affecting angiogenesis and blood vessel integrity.

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1 product image

  • Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (ab234278), expandable thumbnail

    Flow Cytometry - APC Anti-DEP1 antibody [MEM-CD148/05] (ab234278)

    Flow cytometric analysis of human peripheral blood labeling DEP1 using ab234278 at 10 μl/106 cells. Surface staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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