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AB95641

APC Anti-EpCAM antibody [G8.8]

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(4 Publications)

Rat Monoclonal EPCAM antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Epcam protein.

View Alternative Names

CD326, Tacstd1, Epcam, Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, Protein 289A, Tumor-associated calcium signal transducer 1, EGP314, mEGP314

1 Images
Flow Cytometry - APC Anti-EpCAM antibody [G8.8] (AB95641)
  • Flow Cyt

Unknown

Flow Cytometry - APC Anti-EpCAM antibody [G8.8] (AB95641)

Flow Cytometry analysis of TE-71 (mouse thymic epithelial stromal cell line) cells labeling EpCAM with : (1) ab95641 at 0.06 μg and (2) 0.06 μg of Allophycocyanin conjugated Rat IgG2a Isotype Control. Total viable cells were used for analysis.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

G8.8

Isotype

IgG2a

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Epcam protein. The exact immunogen used to generate this antibody is proprietary information.

Q99JW5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.125 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p>Final volume of 100 μl.</p><p><a href='/en-us/products/unavailable/rat-igg2a-kappa-monoclonal-klhg2a-1-1-apc-isotype-control-ab129719'>ab129719</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Excitation wavelength: 633 nm Emission wavelength: 660 nm.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
See full target information Epcam
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:2965 PubMed35618699

2022

Single-cell chromatin profiling of the primitive gut tube reveals regulatory dynamics underlying lineage fate decisions.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan J Smith,Hongpan Zhang,Shengen Shawn Hu,Theodora Yung,Roshane Francis,Lilian Lee,Mark W Onaitis,Peter B Dirks,Chongzhi Zang,Tae-Hee Kim

STAR protocols 3:101351 PubMed35516847

2022

Analysis of mouse intestinal organoid culture with conditioned media isolated from mucosal enteric glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meryem B Baghdadi,Tae-Hee Kim

Developmental cell 52:647-658.e6 PubMed32155439

2020

Hedgehog-Activated Fat4 and PCP Pathways Mediate Mesenchymal Cell Clustering and Villus Formation in Gut Development.

Applications

Unspecified application

Species

Unspecified reactive species

Abilasha Rao-Bhatia,Min Zhu,Wen-Chi Yin,Sabrina Coquenlorge,Xiaoyun Zhang,Janghee Woo,Yu Sun,Charlotte H Dean,Aimin Liu,Chi-Chung Hui,Ramesh A Shivdasani,Helen McNeill,Sevan Hopyan,Tae-Hee Kim

International immunology 18:741-53 PubMed16569675

2006

Functional comparison of mouse CIRE/mouse DC-SIGN and human DC-SIGN.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Caminschi,Alexandra J Corbett,Corina Zahra,Mireille Lahoud,Karen M Lucas,Mariam Sofi,David Vremec,Thomas Gramberg,Stefan Pöhlmann,Joan Curtis,Emanuela Handman,Serani L H van Dommelen,Peter Fleming,Mariapia A Degli-Esposti,Ken Shortman,Mark D Wright
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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