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AB315146

APC Anti-ERG antibody [EPR3864]

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Rabbit Recombinant Monoclonal ERG antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

1 Images
Flow Cytometry (Intracellular) - APC Anti-ERG antibody [EPR3864] (AB315146)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-ERG antibody [EPR3864] (AB315146)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab315146 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab315146) (1x 106in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3864

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.
Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
See full target information ERG
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Product promise

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For full details, please see our Terms & Conditions

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