JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB278539

APC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ERK1 phospho T202 + Y204 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAPK3 phospho Y204 + T202.

View Alternative Names

ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

1 Images
Flow Cytometry - APC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278539)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11] (AB278539)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with U0126 (red) or treated with TPA (green) using ab278539.

  • Unconjugated

    Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

  • 519 FITC

    FITC Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

  • 578 PE

    PE Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody [ERK12T202Y204-A11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

ERK12T202Y204-A11

Isotype

IgG

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human MAPK3 phospho Y204 + T202. The exact immunogen used to generate this antibody is proprietary information.

P27361

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 and ERK2 also known as p44 and p42 MAPK respectively are important proteins in the MAP kinase signaling pathway. They are expressed in various tissues with significant presence in the brain lungs and skin. ERK1 has a molecular weight of approximately 44 kDa while ERK2 has a molecular weight of around 42 kDa. Both proteins become activated through phosphorylation which is essential for their function in cellular processes.
Biological function summary

ERK1 and ERK2 serve as key players in cellular growth differentiation and survival. They form part of a complex cascade where they transduce signals from the cell membrane to the nucleus after activation by phosphorylation. This phosphorylation enables them to modify various downstream targets involved in regulating gene expression and cellular response to external stimuli.

Pathways

ERK1 and ERK2 are critical components of the MAPK/ERK pathway and the Ras-Raf-MEK-ERK signaling cascade. These pathways regulate a multitude of cellular activities including proliferation and differentiation. In the MAPK/ERK pathway proteins like Ras and Raf serve as upstream activators of ERK1 and ERK2. Both ERK1 and ERK2 also interact with other signaling proteins such as MEK1/2 which directly phosphorylates and activates them.

Dysregulation of ERK1 and ERK2 is associated with various pathologies including cancer and neurodegenerative diseases. Abnormal activation of these proteins often leads to uncontrolled cell proliferation contributing to oncogenesis. For instance mutations in proteins like Ras which regulate ERK1 and ERK2 can result in continuous activation and lead to tumor formation. Furthermore altered ERK1 and ERK2 signaling is linked to neurodegeneration impacting neuronal survival and function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway (PubMed : 34497368). MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DEPTOR, FRS2 or GRB10) (PubMed : 35216969). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
See full target information MAPK3 phospho T202 + Y204

Additional targets

MAPK1 phospho T185 + Y187
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB176660

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA Kit

SimpleStep

ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA Kit (AB176660)

  • 5
  • 4
Primary Antibodies

AB278759

APC Anti-MEK1 + MEK2 (phospho S218 + S222) antibody [MEK12S217S221-H2]

Recombinant

Flow Cytometry - APC Anti-MEK1 + MEK2 (phospho S218 + S222) antibody [MEK12S217S221-H2] (AB278759)

  • 0
  • 0
Primary Antibodies

AB278589

APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

Recombinant

Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278589)

  • 0
  • 0
Primary Antibodies

AB184699

Anti-ERK1 + ERK2 antibody [EPR17526]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (AB184699)

  • 5
  • 1
Primary Antibodies

AB201015

Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] (AB201015)

  • 4
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com