AB105080

APC Anti-F4/80 antibody [CI:A3-1]

Name: Anti-F4/80 APC antibody [CI:A3-1] (ab105080) Rat monoclonal IgG2b | Abcam
Brand: Abcam Limited
SKU: AB105080
Price: 430 USD
Availability: InStock

Be the first to review this product! Submit a review

(7 Publications)

Rat Monoclonal F4/80 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing Adgre1 protein.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

2 Images

Flow Cytometry - APC Anti-F4/80 antibody [CI:A3-1] (AB105080)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-F4/80 antibody [CI:A3-1] (AB105080)

Flow cytometry staining of mouse peritoneal macrophages cells with ab105080.

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-F4/80 antibody [CI:A3-1] (AB105080)

FSC/SSC gated mouse peritoneal macrophages dual stained with ab105080 at 1/5 dilution and anti-CD169 at a neat dilution by Flow cytometry. Isotype control pair in red. Fc receptors were blocked.

Unconjugated

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
578 PE

PE Anti-F4/80 antibody [CI:A3-1]
519 FITC

FITC Anti-F4/80 antibody [CI:A3-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CI:A3-1

Isotype

IgG2b

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61549

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>of Neat - 1/10 diluted antibody in 100μl.</p><p><a href='/en-us/products/primary-antibodies/apc-rat-igg2b-kappa-monoclonal-klh-g2b-1-2-isotype-control-ab154434'>ab154434</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 14:3983-4000 PubMed39309491

2024

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Li,Riqing Wei,Chenxin Liu,Keying Zhang,Sixiao He,Zhifeng Liu,Junhao Huang,Youyong Tang,Qiyuan An,Ligen Lin,Lishe Gan,Liying Zhao,Xiaoming Zou,Fudi Wang,Yuan Ping,Qiang Ma

Journal of nanobiotechnology 22:540 PubMed39237942

2024

Enhanced osteogenic differentiation in 3D hydrogel scaffold via macrophage mitochondrial transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Qiu,Lili Cao,Dingding Xiang,Shu Wang,Di Wang,Yiyi Qian,Xiaohua Li,Xiaoshu Zhou

Cancer cell international 21:58 PubMed33468159

2021

IRF4 overexpression promotes the transdifferentiation of tregs into macrophage-like cells to inhibit the development of colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Wang,Song Li,Honglang Li,Xiaoshuang Zhou,Huabin Wen,Bin Lai

Cells 9: PubMed32059476

2020

Unique Pro-Inflammatory Response of Macrophages during Apoptotic Cancer Cell Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Mendoza-Reinoso,Dah Youn Baek,Adrianne Kurutz,John R Rubin,Amy J Koh,Laurie K McCauley,Hernan Roca

Journal of dental research 98:459-467 PubMed30626255

2019

Clodronate-Loaded Liposome Treatment Has Site-Specific Skeletal Effects.

Applications

Unspecified application

Species

Unspecified reactive species

M N Michalski,L E Zweifler,B P Sinder,A J Koh,J Yamashita,H Roca,L K McCauley

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 32:3730-3741 PubMed29475373

2018

Inflammatory bone loss associated with MFG-E8 deficiency is rescued by teriparatide.

Applications

Unspecified application

Species

Unspecified reactive species

Megan N Michalski,Anna L Seydel,Erica M Siismets,Laura E Zweifler,Amy J Koh,Benjamin P Sinder,J Ignacio Aguirre,Kamran Atabai,Hernan Roca,Laurie K McCauley

The Journal of biological chemistry 288:28869-80 PubMed23963447

2013

Microfibril-associated glycoprotein 2 (MAGP2) loss of function has pleiotropic effects in vivo.

Applications

Flow Cyt

Species

Mouse

Michelle D Combs,Russell H Knutsen,Thomas J Broekelmann,Holly M Toennies,Thomas J Brett,Chantel A Miller,Daniel L Kober,Clarissa S Craft,Jeffrey J Atkinson,J Michael Shipley,Barbara C Trask,Robert P Mecham

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-F4/80 antibody [CI:A3-1]

Flow Cytometry - APC Anti-F4/80 antibody [CI:A3-1] (AB105080)

Flow Cytometry - APC Anti-F4/80 antibody [CI:A3-1] (AB105080)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise