AB200568

APC Anti-FOXP3 antibody [3G3]

Name: Anti-FOXP3 APC antibody [3G3] (ab200568) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB200568
Price: 160 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal FOXP3 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Foxp3.

View Alternative Names

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

1 Images

Flow Cytometry (Intracellular) - APC Anti-FOXP3 antibody [3G3] (AB200568)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-FOXP3 antibody [3G3] (AB200568)

Flow Cytometry (Intracellular) analysis of human peripheral blood cells (gated on CD4+ cells) labelingFOXP3 with ab200568 at 2 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G3

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Foxp3.

Q99JB6

Epitope

The mouse monoclonal antibody 3G3 recognizes the N-terminal region of FOXP3.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-2 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab200568 is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the FOXP3 gene is a transcriptional regulator critical for the development and inhibitory function of regulatory T-cells (Treg). It plays an essential role in immune system homeostasis by ensuring the suppressive function and stability of Treg cells and directly influencing conventional T-cells' expansion and function. FOXP3 can function as either a transcriptional repressor or activator, depending on its interactions with other factors, such as transcription factors, histone acetylases, and deacetylases. It co-activates genes like CTLA4 and TNFRSF18 and represses cytokine genes, including interleukin-2 (IL2) and interferon-gamma (IFNG), and inhibits cytokine production by repressing the activity of transcription factors RELA and NFATC2. FOXP3's association with histone acetylase KAT5 and deacetylase HDAC7 mediates IL2 repression, while its interaction with transcription factor RUNX1 modulates the expression of TNFRSF18, IL2RA, CTLA4, IL2, and IFNG. It also antagonizes RORC to inhibit the differentiation of IL17 producing helper T-cells (Th17), leading to reduced IL17 expression and promoting Treg development, while inhibiting the transcriptional activator activity of RORA. This supplementary information is collated from multiple sources and compiled automatically.

See full target information FOXP3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2022:8890434 PubMed35664563

2022

Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4CD25FOXP3 Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Renyong Wang,Ruixue Li,Tiehan Li,Lei Zhu,Zongze Qi,Xiaokui Yang,Huan Wang,Baoquan Cao,Hong Zhu

BMC endocrine disorders 21:57 PubMed33781239

2021

Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wei,Jianhong Jing,Zhiping Peng,Xiaoqian Liu,Xueyang Wang

European journal of immunology 49:1278-1290 PubMed31054264

2019

Engineering antigen-specific NK cell lines against the melanoma-associated antigen tyrosinase via TCR gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Ayhan Parlar,Ece Canan Sayitoglu,Didem Ozkazanc,Anna-Maria Georgoudaki,Cevriye Pamukcu,Mertkaya Aras,Benjamin J Josey,Michael Chrobok,Suzanne Branecki,Pegah Zahedimaram,Lolai Ikromzoda,Evren Alici,Batu Erman,Adil D Duru,Tolga Sutlu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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APC Anti-FOXP3 antibody [3G3]

Flow Cytometry (Intracellular) - APC Anti-FOXP3 antibody [3G3] (AB200568)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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