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AB223949

APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

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(2 Publications)

Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin, GEC1, GABARAPL1, Gamma-aminobutyric acid receptor-associated protein-like 1, Early estrogen-regulated protein, GABA(A) receptor-associated protein-like 1, Glandular epithelial cell protein 1, GEC-1, FLC3A, GEF2, GABARAPL2, Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, MAP1 light chain 3-related protein, GEF-2, GATE-16

1 Images
Flow Cytometry (Intracellular) - APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223949)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223949)

Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab223949 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223949, 1/500 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • 578 PE

    PE Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • HRP

    HRP Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • Unconjugated

    Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805]

  • Carrier free

    Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4805

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab223949 will also recognize related targets GABARAPL1 and GABARAPL2: GABARAPL1: pBLAST 100% homology with immunogen. GABARAPL2: pBLAST 93% homology with immunogen.

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Complementary Products

Assay Kits

AB181423

Human Beta-2-Microglobulin ELISA Kit

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ELISA - Human Beta-2-Microglobulin ELISA Kit (AB181423)

  • 0
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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

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Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

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Primary Antibodies

AB18528

Anti-LAMP2A antibody - Lysosome Marker

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2A antibody - Lysosome Marker (AB18528)

  • 4
  • 15
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 13
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).
See full target information B2M

Additional targets

GABARAPL2,GABARAPL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113287 PubMed40894917

2025

Conserved LIR-specific interaction of Sigma-1 receptor and GABARAP.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Wilhelm Baeken,Maximilian Christ,Daniel Schmitt,Wencke Trein,Heike Nagel,Albrecht Martin Clement,Hagen Körschgen,Christian Behl

EMBO reports 23:e53065 PubMed36215690

2022

Lipid and protein content profiling of isolated native autophagic vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schmitt,Süleyman Bozkurt,Pascale Henning-Domres,Heike Huesmann,Stefan Eimer,Laura Bindila,Christian Behrends,Emily Boyle,Florian Wilfling,Georg Tascher,Christian Münch,Christian Behl,Andreas Kern
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com