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Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (AB223949), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/500
Notes

The cellular localisation of this product has been verified in ICC/IF.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).

Additional Targets

GABARAPL2, GABARAPL1

Alternative names

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

Recommended products

Rabbit Recombinant Monoclonal beta 2 Microglobulin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4805
Purification technique
Affinity purification Protein A
Specificity

ab223949 will also recognize related targets GABARAPL1 and GABARAPL2:

GABARAPL1: pBLAST 100% homology with immunogen.
GABARAPL2: pBLAST 93% homology with immunogen.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab223949), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Anti-GABARAP+GABARAPL1+GABARAPL2 antibody [EPR4805] (ab223949)

    Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab223949 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223949, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com