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AB306251

APC Anti-Gephyrin antibody [EPR12651(B)]

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Rabbit Recombinant Monoclonal Gephyrin antibody - conjugated to APC.

View Alternative Names

GPH, KIAA1385, GPHN, Gephyrin

  • Unconjugated

    Anti-Gephyrin antibody [EPR12651(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Gephyrin antibody [EPR12651(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Gephyrin antibody [EPR12651(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Gephyrin antibody [EPR12651(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Gephyrin antibody [EPR12651(B)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Gephyrin antibody [EPR12651(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Gephyrin antibody [EPR12651(B)]

  • Carrier free

    Anti-Gephyrin antibody [EPR12651(B)] - BSA and Azide free

  • HRP

    HRP Anti-Gephyrin antibody [EPR12651(B)]

  • 578 PE

    PE Anti-Gephyrin antibody [EPR12651(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12651(B)

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gephyrin also known as GPHN is a multifunctional protein integral to synaptic transmission. Weighing approximately 93 kDa it facilitates the anchoring of inhibitory neurotransmitter receptors at postsynaptic sites. This protein is expressed primarily in central nervous system tissues including the brain and spinal cord. Gephyrin performs its mechanical role by forming a scaffold to maintain GABAergic and glycinergic receptors' proper positioning and function.
Biological function summary

Gephyrin's function extends beyond receptor clustering. It plays a part in the larger postsynaptic density complex. This scaffolding capability allows gephyrin to organize inhibitory synapses critical for maintaining neuronal excitability and plasticity. The protein contributes by tethering receptors to the cytoskeleton which stabilizes synaptic structures and allows dynamic interactions with other proteins in the synapse.

Pathways

Gephyrin interacts with the inhibitory signaling pathway. This pathway includes important proteins like GABA receptors and glycine receptors which mediate inhibitory synaptic transmission in the central nervous system. Gephyrin's interactions facilitate the formation of gephyrin clusters which influence synaptic inhibition by crosslinking with tubulin and microtubules significantly impacting neuronal signaling.

Disruptions in gephyrin function link to neurological conditions such as epilepsy and schizophrenia. Mutations or alterations in gephyrin expression affect receptor clustering which impacts synapse formation and can result in dysfunctional neural networks. In schizophrenia gephyrin associates with other synaptic proteins such as neuroligin and neurexins indicating potential contributions to synaptic abnormalities seen in the disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed : 25025157, PubMed : 26613940).. Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released.
See full target information GPHN
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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