Rabbit Recombinant Monoclonal GFP antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Tag samples.
Images
![Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (AB283597), expandable thumbnail](https://content.abcam.com/products/images/apc-gfp-antibody-epr14104-ab283597--flow-cytometry-intracellular-img173153.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (AB283597)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- APC
- Excitation/Emission
- Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Tag | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
Target data
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Alternative names
Green fluorescent protein, GFP
Recommended products
Rabbit Recombinant Monoclonal GFP antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Tag samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- APC
- Excitation/Emission
- Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR14104
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (ab283597), expandable thumbnail](https://content.abcam.com/products/images/apc-gfp-antibody-epr14104-ab283597--flow-cytometry-intracellular-img173153.jpg "Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (ab283597)")
Flow Cytometry (Intracellular) - APC Anti-GFP antibody [EPR14104] (ab283597)
Flow cytometry staining of HEK-293T cells transfected with GFP stained with ab283597 (right) or Rabbit IgG (monoclonal) Allophycocyanin (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) isotype control (left) . The cells were fixed with 4 % formaldehyde (10 min) and then permeabilized with 90% Methanol. The cells were then incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab283597) (1x 106 in 100μl at 1μg/ml (1/500)) for 30 min at 22°C.
Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 670/14 bandpass filter.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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