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AB319219

APC Anti-GRSF1 antibody [EPR16678]

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Rabbit Recombinant Monoclonal GRSF1 antibody - conjugated to APC.

View Alternative Names

G-rich sequence factor 1, GRSF-1, GRSF1

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16678

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The G-rich RNA sequence binding factor 1 known as GRSF1 is a protein involved in RNA processing activities. Alternate names include MGC60760 and GAR1. This protein weighs approximately 48 kDa and demonstrates a wide expression pattern across different tissues with a marked presence in mitochondria. GRSF1 binds specifically to G-rich elements in RNA playing a mechanical role in stabilizing complex RNA structures and facilitating efficient RNA processing.
Biological function summary

The GRSF1 operates as an important player in mitochondrial RNA metabolism. It is known to interact within ribonucleoprotein complexes facilitating the regulation of mitochondrial gene expression. GRSF1 contributes to the maturation and processing of mitochondrial RNA impacting the proper synthesis of components essential for mitochondrial function. It ensures that mitochondrial encoded proteins are synthesized accurately aiding overall cellular energy production.

Pathways

GRSF1 engagement with organellar RNA processing integrates into mitochondrial biogenesis and maintenance pathways. This protein significantly influences the electron transport chain by ensuring the precise maturation of mitochondrial RNAs required for encoding electron transport chain components. It connects with proteins like LRPPRC as part of RNA granules coordinating gene expression regulation to support cellular respiration and energy conversion pathways.

GRSF1 links closely to conditions involving mitochondrial dysfunctions such as mitochondrial myopathy. Alterations in GRSF1 function can result in impaired energy production leading to muscle weakness and other systemic symptoms associated with myopathy. It may also intersect with neurodegenerative disorders where mitochondrial impairment represents a common pathological feature. GRSF1's association with proteins like Twinkle helicase in such diseases emphasizes its broader implications in maintaining mitochondrial integrity and cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences (PubMed : 23473033, PubMed : 23473034, PubMed : 29967381). Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PubMed : 29967381). Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome (PubMed : 29967381). G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA (PubMed : 29967381).
See full target information G-rich sequence factor 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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