Rabbit Recombinant Monoclonal Huntingtin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
APC
Ex: 650nm, Em: 660nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
Flow Cyt (Intra) | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes The cellular localisation of this product has been verified in ICC/IF. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Select an associated product type
HuntingtinMay play a role in microtubule-mediated transport or vesicle function.Huntingtin, myristoylated N-terminal fragmentPromotes the formation of autophagic vesicles.
HD, IT15, IT15, HD, HTT, Huntingtin, Huntington disease protein, HD protein
Rabbit Recombinant Monoclonal Huntingtin antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
APC
Ex: 650nm, Em: 660nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Liquid
Monoclonal
EP867Y
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Upon delivery aliquot
Do Not Freeze, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Overlay histogram showing SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained with ab224969 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab224969, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab232814) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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