APC Anti-IDH2 antibody [EPR7577]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - APC Anti-IDH2 antibody [EPR7577] (AB317932)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat IDH2 knockout cells labeling IDH2 with ab317932 (green) at 0.2 μg/ml. Cells were counterstained with ab195887 Alexa Fluor® 488 Anti-alpha Tubulin [DM1A] at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (Blue).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-IDH2 antibody [EPR7577] (AB317932)

Flow cytometry overlay histogram showing wild-type Jurkat (green line) and IDH2 knockout Jurkat stained with ab317932 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab317932) (1x 10⁶ in 100μl at 0.008 μg/ml (1/62500)) for 30min at 22°C.

Isotype control antibody was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control in Jurkat WT cells (black line) and Jurkat-IDH2 KO cells (grey line), used at the same concentration and conditions as the primary antibody.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.