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AB326455

APC Anti-IL-2 Receptor alpha antibody [MEM-181]

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Mouse Monoclonal IL2RA antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

5 Images
Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)

Separation of CD3 positive CD25 positive lymphocytes (red-filled) from CD3 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral whole blood stained using ab326455 (concentration in sample 0.5 μg/ml).

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)

Flow cytometry multicolor surface staining pattern of human lymphocytes using ab326455 (concentration in sample 0.5 μg/ml) and anti-human CD4 (MEM-241) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)

Separation of CD4 positive CD25 positive Treg cells (red-filled) from CD4 negative CD25 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326455 (concentration in sample 0.5 μg/ml).

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)

Flow cytometry multicolor surface staining pattern of human PHA stimulated lymphocytes using ab326455 (concentration in sample 0.5 μg/ml) and anti-human CD3 (UCHT1) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-IL-2 Receptor alpha antibody [MEM-181] (AB326455)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326455 (concentration in sample 0.5 μg/ml).

  • Unconjugated

    Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 785 APC/Cy7®

    APC/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 519 FITC

    FITC Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 578 PE

    PE Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 778 PE/Cy7®

    PE/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-IL-2 Receptor alpha antibody [MEM-181]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-181

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information IL2RA
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Product promise

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For full details, please see our Terms & Conditions

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