AB41460

APC Anti-Integrin alpha 2 antibody [DX5]

Name: Anti-Integrin alpha 2 APC antibody [DX5] (ab41460) IgM | Abcam
Brand: Abcam Limited
SKU: AB41460
Price: 265 USD
Availability: InStock

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(3 Publications)

Rat Monoclonal Integrin alpha 2 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Itga2 protein.

View Alternative Names

CD49b, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa, Itga2

1 Images

Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (AB41460)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (AB41460)

Flow Cytometry analysis of C57BL/6 mouse splenocytes stained for Integrin alpha 2 using ab41460 (0.1 μg/10⁶ cells) and a Mouse Anti-Mouse NK1.1-PE.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

DX5

Isotype

IgM

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itga2 protein. The exact immunogen used to generate this antibody is proprietary information.

Q62469

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Reactivity data

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Product details

This monoclonal antibody, clone DX5, reacts with CD49b, also known as very late antigen-2 (VLA-2) and Integrin alpha 2. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes.2 NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.

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Properties and storage information

Form

Liquid

Purity

IgM fraction

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis.

See full target information Itga2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 6:1053-9 PubMed21720318

2011

Reproducible subcutaneous transplantation of cell sheets into recipient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Haruko Obokata,Masayuki Yamato,Satoshi Tsuneda,Teruo Okano

Journal of immunology (Baltimore, Md. : 1950) 167:1141-4 PubMed11466327

2001

Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2).

Applications

Unspecified application

Species

Unspecified reactive species

H Arase,T Saito,J H Phillips,L L Lanier

Journal of immunology (Baltimore, Md. : 1950) 157:2366-73 PubMed8805634

1996

Inhibition of gamma delta T cell development and early thymocyte maturation in IL-7 -/- mice.

Applications

Unspecified application

Species

Unspecified reactive species

T A Moore,U von Freeden-Jeffry,R Murray,A Zlotnik

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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APC Anti-Integrin alpha 2 antibody [DX5]

Flow Cytometry - APC Anti-Integrin alpha 2 antibody [DX5] (AB41460)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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