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AB93539

APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)]

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(3 Publications)

Rat Monoclonal Integrin alpha 4/CD49D antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Itga4 protein.

View Alternative Names

CD49d, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, Lymphocyte Peyer patch adhesion molecules subunit alpha, VLA-4 subunit alpha, LPAM subunit alpha, Itga4, Integrin beta-7, Integrin beta-P, M290 IEL antigen, Itgb7

1 Images
Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (AB93539)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (AB93539)

C57Bl/6 bone marrow cells stained with FITC-conjugated anti-mouse CD45 (B220) and 0.25μg ab93539 (right) or 0.25μg APC-conjugated rat IgG2a isotype control (left).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

DATK32 (DATK-32)

Isotype

IgG2a

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Itga4 protein. The exact immunogen used to generate this antibody is proprietary information.

Q00651

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Alternative Products

Primary Antibodies

AB95788

PE Anti-Integrin alpha 4+beta 7 antibody [DATK32]

Flow Cytometry - PE Anti-Integrin alpha 4+beta 7 antibody [DATK32] (AB95788)

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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  • 16
Primary Antibodies

AB19580

Anti-CD18 antibody [C71/16]

Recombinant|Trial Size

Flow Cytometry - Anti-CD18 antibody [C71/16] (AB19580)

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Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

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Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.88% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 beta 7 also known as alpha 4 beta 7 integrin A4B7 integrin and beta-7 is a cell surface integrin receptor involved in mediating cell adhesion. This target plays a mechanical role in binding to the mucosal addressin cell adhesion molecule-1 (MAdCAM-1) primarily on high endothelial venules in the gut-associated lymphoid tissue. It forms a heterodimer composed of the alpha 4 and beta 7 subunits with an estimated mass of about 150–180 kDa. Integrin alpha 4 beta 7 is expressed on the surface of leukocytes including T cells B cells and other immune cells facilitating their tissue-specific homing.
Biological function summary

Integrin alpha 4 beta 7 serves an important role in the migration and homing of lymphocytes to gut-associated lymphoid tissues ensuring immune surveillance. It is a part of the integrin family complex interacting with MAdCAM-1 to guide lymphocytes across the endothelium into the intestinal lamina propria. This process is essential for maintaining the immune cell balance within the gastrointestinal tract where the immune system encounters numerous antigens.

Pathways

Integrin alpha 4 beta 7 fits into immune cell trafficking and adhesion pathways. It works closely with proteins like MAdCAM-1 and interacts with the Janus kinase-signal transducer and activator of transcription (JAK-STAT) signaling pathway. These interactions reinforce the pivotal role of integrin in the precise targeting and localization of immune cells which is necessary for effective immune responses and homeostasis maintenance within specific tissue environments.

Integrin alpha 4 beta 7 connects with conditions such as inflammatory bowel diseases including Crohn's disease and ulcerative colitis. Its interaction with MAdCAM-1 contributes to the recruitment of inflammatory cells to the gut exacerbating inflammation in these disorders. Moreover integrin alpha 4 beta 7 has been implicated in HIV infection where it serves as a marker for lymphocytes targeted by the virus highlighting its potential as a therapeutic target for modulating immune system activity in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrins alpha-4/beta-1 (VLA-4 or LPAM-2) and alpha-4/beta-7 (LPAM-1) are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information Itga4

Additional targets

Itgb7
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 153:3847-61 PubMed7523506

1994

Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.

Applications

Flow Cyt

Species

Unspecified reactive species

D P Andrew,C Berlin,S Honda,T Yoshino,A Hamann,B Holzmann,P J Kilshaw,E C Butcher

Journal of immunology (Baltimore, Md. : 1950) 152:3282-93 PubMed7511642

1994

Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo.

Applications

Flow Cyt

Species

Unspecified reactive species

A Hamann,D P Andrew,D Jablonski-Westrich,B Holzmann,E C Butcher

Cell 74:185-95 PubMed7687523

1993

Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1.

Applications

Flow Cyt

Species

Unspecified reactive species

C Berlin,E L Berg,M J Briskin,D P Andrew,P J Kilshaw,B Holzmann,I L Weissman,A Hamann,E C Butcher
View all publications
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