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AB303170

APC Anti-IQGAP1 antibody [EPR5220]

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Rabbit Recombinant Monoclonal IQGAP1 antibody - conjugated to APC.

View Alternative Names

KIAA0051, IQGAP1, Ras GTPase-activating-like protein IQGAP1, p195

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-IQGAP1 antibody [EPR5220]

  • 578 PE

    PE Anti-IQGAP1 antibody [EPR5220]

  • HRP

    HRP Anti-IQGAP1 antibody [EPR5220]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-IQGAP1 antibody [EPR5220]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-IQGAP1 antibody [EPR5220]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-IQGAP1 antibody [EPR5220]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5220

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

This conjugated primary antibody is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the Lightning-Link® conjugation technology, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.

For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IQGAP1 also known as IQ motif containing GTPase activating protein 1 acts as a scaffold protein that interacts with several signaling molecules. It weighs approximately 189 kDa. This protein is expressed in various tissues but shows high expression in the brain liver and kidney. IQGAP1 contains multiple domains including four IQ motifs that bind to calmodulin and a Rho GTPase-activating protein-related domain that mediates interactions with GTPases.
Biological function summary

IQGAP1 plays a critical role in cytoskeletal organization cell adhesion and intracellular signaling. It participates in the formation of multiprotein complexes facilitating communication and structural integrity within the cell. IQGAP1 stabilizes the actin cytoskeleton and affects cell motility and polarity. It interacts with proteins such as E-cadherin and beta-catenin affecting cell-cell adhesion and signal transduction.

Pathways

IQGAP1 integrates signals from different pathways including the MAPK/ERK and Wnt pathways. It modulates the MAPK pathway by interacting with Raf and MEK kinases impacting cell proliferation and differentiation. In the Wnt signaling pathway IQGAP1 binds to APC and beta-catenin regulating gene transcription related to cell growth and division. These interactions highlight its role in coordinating cellular responses to various external signals.

IQGAP1 has associations with cancer and neurological disorders. In cancer alterations in IQGAP1 expression or function can lead to abnormal cell growth and metastasis due to disrupted signaling pathways like MAPK. Mutations and dysregulation of proteins such as Ras and beta-catenin commonly interact with IQGAP1 in oncogenic contexts. In neurological disorders its interaction with the actin cytoskeleton and signaling proteins can affect synapse formation and function with links to conditions such as schizophrenia and Alzheimer's disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed : 15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed : 20883816).
See full target information IQGAP1
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Alternative Products

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AB222390

Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] - Low endotoxin, Azide free (AB222390)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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