AB155363

APC Anti-LIGHT/TNFSF14 antibody [7-3 (7)]

Name: Anti-LIGHT/TNFSF14 APC antibody [7-3 (7)] (ab155363) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB155363

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(1 Publication)

Mouse Monoclonal LIGHT/TNFSF14 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human TNFSF14.

View Alternative Names

CD258, HVEML, LIGHT, UNQ391/PRO726, TNFSF14, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, HVEM-L, Herpesvirus entry mediator ligand

1 Images

Flow Cytometry - APC Anti-LIGHT/TNFSF14 antibody [7-3 (7)] (AB155363)

Flow Cyt

Unknown

Flow Cytometry - APC Anti-LIGHT/TNFSF14 antibody [7-3 (7)] (AB155363)

Flow cytometric analysis of normal Human peripheral blood cells with Anti-Human CD8a PE and mouse IgG1 kappa Isotype Control APC (left) or 5 µl (0.25 µg) of ab155363 (right). Total viable cells were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7-3 (7)

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human TNFSF14.

O43557

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIGHT also known as TNFSF14 is a member of the tumor necrosis factor (TNF) superfamily. It has an approximate mass of 24 kDa. You can find LIGHT expressed in various tissues including lymphoid tissues such as the spleen and lymph nodes. LIGHT serves as a ligand that can bind to receptors like lymphotoxin beta receptor (LTβR) and herpesvirus entry mediator (HVEM). Researchers have studied "mouse LIGHT" and "LIGHT protein" to understand its role in immune regulation. Additionally the term "anti-LIGHT" often refers to tools like antibodies used to investigate or inhibit LIGHT's activity.

Biological function summary

LIGHT plays a role in regulating immune responses and inflammation. It is part of a complex formed when it binds to its receptors influencing the proliferation and survival of immune cells such as T cells. LIGHT's interaction with HVEM can modulate T cell signaling cytokine production and dendritic cell maturation. It contributes to the organization of secondary lymphoid organs impacting immune surveillance and homeostasis. The presence of "APC LIGHT" indicates its involvement in antigen-presenting cell activities essential for adaptive immunity.

Pathways

LIGHT operates within the TNF receptor superfamily signaling pathway. This pathway involves other TNF members like TNF-α and lymphotoxin alfa which can also interact with LTβR. Another relevant pathway is the NF-kB signaling cascade which LIGHT activation can trigger. This cascade regulates gene expression related to immune response and cell survival. In connection to these pathways LIGHT's interaction with proteins like HVEM and LTβR helps maintain immune system balance.

LIGHT has links to autoimmune diseases and cancer. Abnormal LIGHT signaling can contribute to conditions like rheumatoid arthritis where immune cell regulation becomes dysregulated. In cancer LIGHT may play a dual role by promoting tumor immunity or conversely tumor progression depending on the context of its expression and receptor interaction. The LIGHT interaction network includes TNFRSF members and can influence pathological inflammatory responses when disrupted.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM (PubMed : 10754304, PubMed : 9462508). Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production (PubMed : 10754304).

See full target information TNFSF14

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 4:102053 PubMed36853720

2023

In vitro expansion of Wilms' tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sanne van der Heijden,Donovan Flumens,Maarten Versteven,Stefanie Peeters,Hans De Reu,Diana Campillo-Davo,Yannick Willemen,Benson Ogunjimi,Viggo Van Tendeloo,Zwi N Berneman,Sébastien Anguille,Evelien Smits,Eva Lion

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-LIGHT/TNFSF14 antibody [7-3 (7)]

Flow Cytometry - APC Anti-LIGHT/TNFSF14 antibody [7-3 (7)] (AB155363)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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