JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB303606

APC Anti-Ly6g antibody [EPR22909-135]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Ly6g antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

1 Images
Flow Cytometry - APC Anti-Ly6g antibody [EPR22909-135] (AB303606)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-Ly6g antibody [EPR22909-135] (AB303606)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab303606 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti CD16/CD32 and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab303606) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) isotype (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30 mins on ice. The cells were simultaneously stained with CD11b.

Acquisition of >30000 events were collected using a 40 mW Red laser (638 nm) and 660/10 bandpass filter. Events were gated on live monocytes.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Ly6g antibody [EPR22909-135]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

  • 578 PE

    PE Anti-Ly6g antibody [EPR22909-135]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Ly6g antibody [EPR22909-135]

  • 519 FITC

    FITC Anti-Ly6g antibody [EPR22909-135]

  • Unconjugated

    Anti-Ly6g antibody [EPR22909-135]

  • Carrier free

    Anti-Ly6g antibody [EPR22909-135] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22909-135

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Ly6g
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB319187

Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23]

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23] (AB319187)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB307523

Alexa Fluor® 647 Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 647 Anti-CD11b antibody [EPR1344] (AB307523)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com