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AB25273

APC Anti-Ly6g antibody [RB6-8C5]

4

(6 Reviews)

|

(2 Publications)

Rat Monoclonal Ly6g antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g, Lymphocyte antigen 6C1, Ly-6C1, Ly6c1

1 Images
Flow Cytometry - APC Anti-Ly6g antibody [RB6-8C5] (AB25273)
  • Flow Cyt

Unknown

Flow Cytometry - APC Anti-Ly6g antibody [RB6-8C5] (AB25273)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labelling Ly6g with ab25273 at 1.125 ug (solid line) compared to a APC Rat IgG2b isotype control (dashed line) at 0.125 ug

  • Unconjugated

    Anti-Ly6g + Ly6c antibody [RB6-8C5]

  • 519 FITC

    FITC Anti-Ly6g antibody [RB6-8C5]

  • 578 PE

    PE Anti-Ly6g antibody [RB6-8C5]

  • 778 PE/Cy7®

    PE/Cy7® Anti-Ly6g antibody [RB6-8C5]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

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Alternative Products

Primary Antibodies

AB25680

Biotin Anti-Ly6g antibody [RB6-8C5]

Flow Cytometry - Biotin Anti-Ly6g antibody [RB6-8C5] (AB25680)

  • 0
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Primary Antibodies

AB210201

APC Anti-Ly6g antibody [1A8]

Flow Cytometry - APC Anti-Ly6g antibody [1A8] (AB210201)

  • 0
  • 0
Primary Antibodies

AB210204

Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free

Flow Cytometry - Anti-Ly6g antibody [1A8] - Low endotoxin, Azide free (AB210204)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB319187

Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23]

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6c antibody [EPR27220-23] (AB319187)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB307523

Alexa Fluor® 647 Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 647 Anti-CD11b antibody [EPR1344] (AB307523)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.25 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 16% Sucrose
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.
Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ly6g

Additional targets

Ly6c1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112352 PubMed37027297

2023

BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica R Doll,Maria E Moreno-Fernandez,Traci E Stankiewicz,Jennifer L Wayland,Adrienne Wilburn,Benjamin Weinhaus,Claire A Chougnet,Daniela Giordano,Monica Cappelletti,Pietro Presicce,Suhas G Kallapur,Nathan Salomonis,Tamara Tilburgs,Senad Divanovic

HPB : the official journal of the International He 21:1344-1353 PubMed30879992

2019

Normal and fibrotic liver parenchyma respond differently to irreversible electroporation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenang Lyu,Maya Lopez-Ichikawa,Boris Rubinsky,Tammy T Chang
View all publications
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Product promise

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