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Rabbit Recombinant Monoclonal MEK3 phospho S189 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP2K3 phospho S189.

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Images

Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278589), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human MAP2K3 phospho S189. The exact immunogen used to generate this antibody is proprietary information. Database link P46734

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µL for 106 Cells
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.

Additional Targets

MAP2K6 phospho S207

Alternative names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

Recommended products

Rabbit Recombinant Monoclonal MEK3 phospho S189 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP2K3 phospho S189.

Key facts

Isotype
IgG
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogens
  • Synthetic Peptide within Human MAP2K3 phospho S189. The exact immunogen used to generate this antibody is proprietary information. Database link P46734
Clone number
MKK3S189MKK6S207-D3
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MEK3 also known as MAP2K3 and MKK6 known as MAP2K6 are protein kinases involved in the MAP kinase signaling pathway. MEK3 has a molecular weight of approximately 38 kDa and MEK6 has a similar molecular weight. Researchers have identified these kinases in various human tissues with significant expression in muscles heart and brain tissues. Both MEK3 and MKK6 activate specific downstream MAP kinases via phosphorylation facilitating their role in cellular stress responses.

Biological function summary

MEK3 and MKK6 mediate responses to stress and inflammatory cytokines marking their importance in signal transduction. They activate p38 MAP kinase through phosphorylation a part of a complex necessary for cellular responses. This activity influences gene expression related to cellular growth differentiation and apoptosis. MEK3 and MKK6 show functional overlap and redundancy ensuring robust cellular responses to external stimuli.

Pathways

MEK3 and MKK6 are integral in the MAP kinase signaling pathway and the p38 MAPK pathway. These pathways play critical roles in cell differentiation apoptosis and response to extracellular stress signals. MEK3 and MEK6 phosphorylate and activate the p38 MAPK protein which regulates stress-induced gene expression. This relationship emphasizes the interplay between kinases and how MEK3 and MEK6 fit within broader signaling networks.

Associated diseases and disorders

MEK3 and MKK6 are associated with inflammatory diseases and cancer. Dysregulation of the p38 MAPK pathway involving MEK3 and MKK6 contributes to these conditions due to improper stress response signaling. Notably MEK6's interaction with the p38 protein highlights its potential role in exacerbating inflammation and tumorigenesis. Understanding the mechanisms involving MEK3 and MKK6 assists in the development of targeted therapies for these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (ab278589), expandable thumbnail

    Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (ab278589)

    Flow cytometric analysis of HEK293T cells K252a treated cells as negative control (blue) or stained and treated with K252a (red) or treated with UV+TPA (green) using ab278589.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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