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AB278589

APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

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Rabbit Recombinant Monoclonal MEK3 phospho S189 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP2K3 phospho S189.

View Alternative Names

MEK3, MKK3, PRKMK3, SKK2, MAP2K3, Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, MEK 3, SAPK kinase 2, SAPKK-2, SAPKK2

1 Images
Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278589)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3] (AB278589)

Flow cytometric analysis of HEK293T cells K252a treated cells as negative control (blue) or stained and treated with K252a (red) or treated with UV+TPA (green) using ab278589.

  • Unconjugated

    Anti-MEK 3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

  • 578 PE

    PE Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

  • 519 FITC

    FITC Anti-MEK3 (phospho S189) + MKK6 (phospho S207) antibody [MKK3S189MKK6S207-D3]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MKK3S189MKK6S207-D3

Isotype

IgG

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human MAP2K3 phospho S189. Database link P46734

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK3 also known as MAP2K3 and MKK6 known as MAP2K6 are protein kinases involved in the MAP kinase signaling pathway. MEK3 has a molecular weight of approximately 38 kDa and MEK6 has a similar molecular weight. Researchers have identified these kinases in various human tissues with significant expression in muscles heart and brain tissues. Both MEK3 and MKK6 activate specific downstream MAP kinases via phosphorylation facilitating their role in cellular stress responses.
Biological function summary

MEK3 and MKK6 mediate responses to stress and inflammatory cytokines marking their importance in signal transduction. They activate p38 MAP kinase through phosphorylation a part of a complex necessary for cellular responses. This activity influences gene expression related to cellular growth differentiation and apoptosis. MEK3 and MKK6 show functional overlap and redundancy ensuring robust cellular responses to external stimuli.

Pathways

MEK3 and MKK6 are integral in the MAP kinase signaling pathway and the p38 MAPK pathway. These pathways play critical roles in cell differentiation apoptosis and response to extracellular stress signals. MEK3 and MEK6 phosphorylate and activate the p38 MAPK protein which regulates stress-induced gene expression. This relationship emphasizes the interplay between kinases and how MEK3 and MEK6 fit within broader signaling networks.

MEK3 and MKK6 are associated with inflammatory diseases and cancer. Dysregulation of the p38 MAPK pathway involving MEK3 and MKK6 contributes to these conditions due to improper stress response signaling. Notably MEK6's interaction with the p38 protein highlights its potential role in exacerbating inflammation and tumorigenesis. Understanding the mechanisms involving MEK3 and MKK6 assists in the development of targeted therapies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
See full target information MAP2K3 phospho S189
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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