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AB239462

APC Mouse IgG1 kappa [MOPC-21] - Isotype Control

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APC Mouse IgG1 kappa [MOPC-21] - Isotype Control Suitable for Flow Cyt and reacts with samples.

View Alternative Names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

1 Images
Flow Cytometry - APC Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239462)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Mouse IgG1 kappa [MOPC-21] - Isotype Control (AB239462)

Flow cytometric analysis of Human peripheral whole blood cells using ab239462 at 15 μg/ml.

  • Unconjugated

    Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control

  • 519 FITC

    FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • Biotin

    Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Mouse IgG1, Kappa Monoclonal [MOPC-21] - Isotype Control

  • 785 APC/Cy7®

    APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control

  • 778 PE/Cy7®

    PE/Cy7® Mouse IgG1 kappa Isotype Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MOPC-21

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Flow Cyt

applications

Specificity

ab239462 has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab239462 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.
See full target information IGHG4

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