AB223896

APC Anti-Myc tag antibody [9E10]

Name: Anti-Myc tag APC antibody [9E10] (ab223896) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB223896
Price: 390 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal C-Myc/MYC antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Tag samples. Cited in 2 publications.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

1 Images

Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (AB223896)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (AB223896)

Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223896 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Allophycocyanin (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Allophycocyanin conjugated Myc-tag antibody (ab223896) and Alexa Fluor^® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Myc tag antibody [9E10]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Myc tag antibody [9E10]
HRP

HRP Anti-Myc tag antibody [9E10]
578 PE

PE Anti-Myc tag antibody [9E10]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Myc tag antibody [9E10]
Biotin

Biotin Anti-Myc tag antibody [9E10] - C-terminal
519 FITC

FITC Anti-Myc tag antibody [9E10]
Biotin

Biotin Anti-Myc tag antibody [9E10]
657 SureLight® APC

SureLight® APC Anti-Myc tag antibody [9E10]
576 DyLight® 550

DyLight® 550 Anti-Myc tag antibody [9E10]
673 DyLight® 650

DyLight® 650 Anti-Myc tag antibody [9E10]
518 DyLight® 488

DyLight® 488 Anti-Myc tag antibody [9E10]
Unconjugated

Anti-Myc tag antibody [9E10]
Carrier free

Anti-Myc tag antibody [9E10] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9E10

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

aa 410-419 of human Myc.

Specificity

This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Myc tag often referred to as the c-Myc or 9E11 is a synthetic peptide label widely used to facilitate protein purification and detection. It's a short sequence of amino acids with an approximate mass not exceeding 1.2 kDa. Researchers commonly use it because of its easy detection through antibodies like anti-Myc or anti-Myc HRP. This tag is usually fused to proteins of interest in various expression systems including bacterial yeast and mammalian cells.

Biological function summary

The Myc tag plays no direct role in cellular processes but enables the study of tagged proteins in complex biological environments. It doesn’t interfere with the function of the protein it tags and is part of no native cellular complex. Instead it acts as a versatile tool for researchers to monitor protein expression levels subcellular localization and interactions. The Myc tag size facilitates easy integration into experimental designs making it compatible with a wide range of applications.

Pathways

The Myc tag helps map the role of the proteins it is attached to in significant cellular pathways. While the tag itself does not engage in pathways it assists in understanding protein interactions and cellular signaling mechanisms like the MAPK/ERK pathway or the PI3K/Akt pathway. By monitoring tagged proteins scientists can investigate how they interact with other pathway components enabling insights into cellular function and regulation.

The Myc tag aids research into conditions such as cancer and neurodegenerative diseases. The tag itself isn’t linked to any diseases; however it is often used to study proteins involved in these disorders like those in the Myc-oncoprotein family. Researchers use it to examine over-expression or mutation of target proteins connected to disease mechanisms enabling the development of therapeutic approaches and understanding pathogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadp3672 PubMed39937915

2025

Listerin promotes α-synuclein degradation to alleviate Parkinson's disease through the ESCRT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Qin,Runyu Cao,Wenjing Cui,Xuemei Bai,Jiahua Yuan,Yuling Zhang,Yaxing Liu,Nan Cao,Na Dong,Min Zhou,Tian Chen,Feng Liu,Wanwei Sun,Yi Zheng,Wei Zhao,Bingyu Liu,Chengjiang Gao

Antibodies (Basel, Switzerland) 13: PubMed39311380

2024

A Novel Tetravalent Bispecific Immune Cell Engager Activates Natural Killer Cells to Kill Cancer Cells without Mediating Fratricide.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Yang,Shahryar Khoshtinat Nikkhoi,Hajar Owji,Geng Li,Mohammad Massumi,Jessica Cervelli,Venu Gopal Vandavasi,Arash Hatefi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-Myc tag antibody [9E10]

Flow Cytometry (Intracellular) - APC Anti-Myc tag antibody [9E10] (AB223896)

Related conjugates and formulations (14)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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