AB28335

APC Anti-NCAM1/CD56 antibody [MEM-188]

Name: APC Anti-NCAM1/CD56 antibody [MEM-188] (ab28335) | Abcam
Brand: Abcam Limited
SKU: AB28335
Price: 110 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications )

Anti-NCAM1/CD56 antibody [MEM-188] conjugated to APC - suitable for flow cytometry, and reacts with human samples. Clone [MEM-188] is a mouse monoclonal antibody cited in 54 publications.

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3 Images

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and ab28335 (10 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab28335 (10 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Separation of human CD56 positive CD3 negative NK cells (red-filled) from CD56 negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab28335 (10 µl reagent / 100 µl of peripheral whole blood).

519 FITC

FITC Anti-NCAM1/CD56 antibody [MEM-188]
578 PE

PE Anti-NCAM1/CD56 antibody [MEM-188]
778 PE/Cy7®

PE/Cy7® Anti-NCAM1/CD56 antibody [MEM-188]
675 PerCP

PerCP Anti-NCAM1/CD56 antibody [MEM-188]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-188

Isotype

IgG2a

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing NCAM1/CD56 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

ab28335 recognises an isoform of NCAM characteristic for leucocytes.Reactivity with other NCAM isoforms has not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10 ul per 100 ul of whole blood. <a href='/en-us/products/primary-antibodies/apc-mouse-igg2a-b12-8-isotype-control-ab91364'>ab91364</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.

Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.

See full target information NCAM1/CD56

Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Journal of proteome research 24:3578-3596 PubMed40526398

2025

Quantitative Proteomics Unveils the Synergistic Effects of Combination Drugs on Cytoskeleton Composition and Autophagy-Mediated Cell Death in Neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Chen Yu,Yi-Chun Kao,Hsin-Yi Chang,Chen-Hao Huang,Wen-Ming Hsu,Hsuan-Cheng Huang,Hsueh-Fen Juan

Folia biologica 34:23-34 PubMed2968928

1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I Hilgert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Anti-NCAM1/CD56 antibody [MEM-188]

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

Flow Cytometry - APC Anti-NCAM1 antibody [MEM-188] (AB28335)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise