JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB267561

APC Anti-OX40L/TNFSF4 antibody [EPR23155-317]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal OX40L/TNFSF4 antibody - conjugated to APC. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

CD252, TXGP1, TNFSF4, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40 ligand, TAX transcriptionally-activated glycoprotein 1, OX40L

1 Images
Flow Cytometry - APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (AB267561)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-OX40L/TNFSF4 antibody [EPR23155-317] (AB267561)

Flow cytometry overlay histogram showing left HUVEC positive cells and right negative HeLa cells stained with ab267561 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab267561) (1x 106 in 100μL at 5μg/mL (1/100)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • 578 PE

    PE Anti-OX40L/TNFSF4 antibody [EPR23155-317]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-OX40L/TNFSF4 antibody [EPR23155-317]

  • Unconjugated

    Anti-OX40L/TNFSF4 antibody [EPR23155-317]

  • Carrier free

    Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free (Capture)

  • Carrier free

    Anti-OX40L/TNFSF4 antibody [EPR23155-317] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23155-317

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB213842

Human TNFSF4 ELISA Kit (OX40L)

Sandwich ELISA - Human TNFSF4 ELISA Kit (OX40L) (AB213842)

  • 0
  • 0
Primary Antibodies

AB209478

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB209478)

  • 0
  • 0
Primary Antibodies

AB68185

Anti-4-1BBL antibody [EPR1172Y]

RabMAb|Recombinant

Immunoprecipitation - Anti-4-1BBL antibody [EPR1172Y] (AB68185)

  • 0
  • 0
Primary Antibodies

AB245756

Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [SP195] - BSA and Azide free (AB245756)

  • 0
  • 0
Primary Antibodies

AB303131

PE Anti-STUB1 / CHIP antibody [EPR4447]

RabMAb|Recombinant

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OX40L also known as TNFSF4 is a member of the tumor necrosis factor (TNF) ligand family. It is a type II transmembrane protein with a molecular weight of about 32 kDa. OX40L is expressed mainly on activated antigen-presenting cells such as B cells dendritic cells and macrophages. The protein interacts specifically with its receptor OX40 (CD134) which is found on activated T cells mainly CD4+ T cells. These interactions are important for the regulation of immune responses.
Biological function summary

OX40L acts as a costimulatory signal for T cells. It does not work alone; it functions as part of the immune complex involved in enhancing T cell proliferation cytokine production and survival. OX40L enhances the immune response making it a critical modulator of immunity. This protein interaction is essential for promoting an effective adaptive immune response influencing T cell memory and maintaining immune system balance.

Pathways

OX40L is involved in the TNF receptor pathway which plays an important role in cell survival and inflammation. It influences the NF-κB signaling pathway which regulates immune response and cell proliferation. OX40L interactions also involve the Akt pathway important for T cell survival and function. The related proteins in these pathways include notably the OX40 protein and CD317 which participate in immune signaling and regulation.

OX40L is implicated in autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The interaction between OX40L and OX40 increases T cell activation contributing to inflammation and disease progression. OX40L's excessive activity has links to these pathologies making it a potential therapeutic target. Another related protein within disease contexts is CD317 connected to immune modulation and control of viral infections and offering further opportunities for targeted treatment strategies involving OX40L.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
See full target information TNFSF4
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com