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AB106166

APC Anti-pan Cytokeratin antibody [C-11]

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(4 Publications)

Mouse Monoclonal Cytokeratin 1 antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing KRT1 protein.

View Alternative Names

KRTA, KRT1, 67 kDa cytokeratin, Cytokeratin-1, Hair alpha protein, Keratin-1, Type-II keratin Kb1, CK-1, K1

2 Images
Flow Cytometry (Intracellular) - APC Anti-pan Cytokeratin antibody [C-11] (AB106166)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-pan Cytokeratin antibody [C-11] (AB106166)

Separation of HeLa cells stained using ab106166 (concentration in sample 5 µg/ml red-filled) from HeLa cells stained using mouse IgG1 isotype control APC antibody (concentration in sample 5 µg/ml same as Cytokeratins APC concentration black-dashed) in flow cytometry analysis (intracellular staining).

Flow Cytometry (Intracellular) - APC Anti-pan Cytokeratin antibody [C-11] (AB106166)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-pan Cytokeratin antibody [C-11] (AB106166)

Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa cells using ab106166

  • Unconjugated

    Anti-pan Cytokeratin antibody [C-11]

  • 578 PE

    PE Anti-pan Cytokeratin antibody [C-11]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-pan Cytokeratin antibody [C-11]

  • Carrier free

    Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-pan Cytokeratin antibody [C-11]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-pan Cytokeratin antibody [C-11]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-pan Cytokeratin antibody [C-11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-11

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04264

Specificity

ab106166 reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Mammals": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: 99% PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary

Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.

Pathways

Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).

Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.
See full target information KRT1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Mucosal immunology 9:1125-36 PubMed26813342

2016

Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.

Applications

Flow Cyt

Species

Cow

J G Cronin,V Kanamarlapudi,C A Thornton,I M Sheldon

Endocrinology 155:1453-65 PubMed24437488

2014

Epithelial and stromal cells of bovine endometrium have roles in innate immunity and initiate inflammatory responses to bacterial lipopeptides in vitro via Toll-like receptors TLR2, TLR1, and TLR6.

Applications

Flow Cyt

Species

Cow

Matthew L Turner,James G Cronin,Gareth D Healey,Iain Martin Sheldon

Research communications in molecular pathology and pharmacology 101:115-26 PubMed9821208

1998

Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

H Hamakawa,T Sumida,H Tanioka,K Sogawa,T Yamada

The Journal of pathology 164:215-24 PubMed1716305

1991

A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.

Applications

Unspecified application

Species

Unspecified reactive species

J Bártek,B Vojtĕsek,Z Stasková,J Bártková,Z Kerekés,A Rejthar,J Kovarík
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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