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AB206967

APC Anti-PD-L1 antibody [28-8]

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(4 Publications)

Rabbit Recombinant Monoclonal PD-L1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Transfected cell line, Human samples. Cited in 4 publications.

View Alternative Names

CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1

1 Images
Flow Cytometry - APC Anti-PD-L1 antibody [28-8] (AB206967)
  • Flow Cyt

Lab

Flow Cytometry - APC Anti-PD-L1 antibody [28-8] (AB206967)

Overlay histogram showing CHO-S (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab206967.

The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab206967, 1/500 dilution) for 30 min at 22°C.

Acquisition of >5,000 events were collected using a 17 mW red Helium-Neon laser (633nm) and 660/20 bandpass filter.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PD-L1 antibody [28-8]

  • 578 PE

    PE Anti-PD-L1 antibody [28-8]

  • 519 FITC

    FITC Anti-PD-L1 antibody [28-8]

  • HRP

    HRP Anti-PD-L1 antibody [28-8]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PD-L1 antibody [28-8]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PD-L1 antibody [28-8]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PD-L1 antibody [28-8]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PD-L1 antibody [28-8] - Extracellular domain

  • Biotin

    Biotin Anti-PD-L1 antibody [28-8] (Detector)

  • Unconjugated

    Anti-PD-L1 antibody [28-8]

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free (Detector)

  • Carrier free

    Anti-PD-L1 antibody [28-8] - BSA and Azide free

  • Carrier free

    Anti-PD-L1 antibody [28-8] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

28-8

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" }, "Transfected cell line": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p><strong>The cellular localisation of this product has been verified in ICC/IF.</strong></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.
Biological function summary

PD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.

Pathways

PD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.

PD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 31399419). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11015443, PubMed : 28813410, PubMed : 28813417, PubMed : 36727298). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 10581077). Can also act as a transcription coactivator : in response to hypoxia, translocates into the nucleus via its interaction with phosphorylated STAT3 and promotes transcription of GSDMC, leading to pyroptosis (PubMed : 32929201).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 28813410, PubMed : 28813417). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
See full target information CD274
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC medicine 22:342 PubMed39183296

2024

Multispectral fluorescence imaging of EGFR and PD-L1 for precision detection of oral squamous cell carcinoma: a preclinical and clinical study.

Applications

Unspecified application

Species

Unspecified reactive species

Nenghao Jin,Yu An,Yu Tian,Zeyu Zhang,Kunshan He,Chongwei Chi,Wei Mu,Jie Tian,Yang Du

Small (Weinheim an der Bergstrasse, Germany) 19:e2301019 PubMed37209021

2023

Engineered Cytokine-Primed Extracellular Vesicles with High PD-L1 Expression Ameliorate Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Lanxing Wang,Chunxiao Qi,Hongmei Cao,Yanwen Zhang,Xing Liu,Lina Qiu,Hang Wang,Lijuan Xu,Zhenzhou Wu,Jianfeng Liu,Shusen Wang,Deling Kong,Yuebing Wang

Cells 12: PubMed36980174

2023

Prognostic Role of Soluble and Extracellular Vesicle-Associated PD-L1, B7-H3 and B7-H4 in Non-Small Cell Lung Cancer Patients Treated with Immune Checkpoint Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Genova,Roberta Tasso,Alessandra Rosa,Giovanni Rossi,Daniele Reverberi,Vincenzo Fontana,Silvia Marconi,Michela Croce,Maria Giovanna Dal Bello,Chiara Dellepiane,Marco Tagliamento,Maria Chiara Ciferri,Lodovica Zullo,Alessandro Fedeli,Angela Alama,Katia Cortese,Chiara Gentili,Eugenia Cella,Giorgia Anselmi,Marco Mora,Giulia Barletta,Erika Rijavec,Francesco Grossi,Paolo Pronzato,Simona Coco

Journal of cellular physiology 234:13145-13156 PubMed30584667

2018

The effects of CCR7 and related signaling pathways on Leishmania major -infected human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yumin Zhao,Zhisheng Dang,Riming Wei,Weifeng Gui,Yishu Zhang,Shigui Chong
View all publications
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Product promise

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