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AB278733

APC Anti-PI3K p85 (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

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(2 Publications)

Rabbit Recombinant Monoclonal PI 3 Kinase p85 alpha phospho Y467 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1 phospho Y467.

View Alternative Names

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha, Phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, p55PIK, PI3-kinase subunit p55-gamma, PtdIns-3-kinase regulatory subunit p55-gamma, PIK3R3

1 Images
Flow Cytometry - APC Anti-PI3K p85 (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278733)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-PI3K p85 (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278733)

Flow cytometric analysis of Ramos cells untreated and unstained as negative control (blue) or untreated (red) or treated with pervanadate (green) and stained using ab278733.

  • Unconjugated

    Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • 519 FITC

    FITC Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • 578 PE

    PE Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PI3KY458-1A11

Isotype

IgG

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human PIK3R1 phospho Y467. The exact immunogen used to generate this antibody is proprietary information.

P27986

Specificity

Recent data suggests this antibody can recognize PI 3 Kinase p85 and p55.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PI 3 Kinase p85 alpha also known as PI3K p85 or PI3-kinase p85 is a regulatory subunit part of the phosphoinositide 3-kinases (PI3Ks) complex which is integral to cellular functions. The p85 alpha form partners with the catalytic subunit to form PI3K p55 known for its significant role in signaling. This subunit exhibits a molecular weight of approximately 85 kDa. It shows expression in various tissues including liver muscle and brain where it aids in cellular communication responding to extracellular signals.
Biological function summary

PI 3 Kinase p85 alpha plays an essential role in intracellular signaling where it functions as part of the PI3K complex. It functions to regulate metabolic processes by interacting with phosphatidylinositol lipids in the cell membrane. PI3K p55 form contributes to cell growth survival and proliferation often collaborating with the catalytic subunit p110 to execute these functions. The phospho PI3K form becomes active upon receiving external growth factors facilitating downstream signaling.

Pathways

The involvement of PI 3 Kinase p85 alpha extends to critical cellular pathways like the PI3K/AKT pathway and the insulin signaling pathway. It engages with proteins such as AKT (protein kinase B) and IRS-1 linking to various cellular functions including metabolism protein synthesis and cellular proliferation. The activity of PI3K p55 form regulates these pathways by influencing enzyme activity and pathways associated with cell survival and apoptosis.

Dysregulation of PI 3 Kinase p85 alpha associates closely with cancers and metabolic disorders like type 2 diabetes. Aberrations in the p55 form link to tumorigenesis due to its role in uncontrolled cell proliferation. PI3K pathway disorders also relate to insulin resistance where the malfunction of PI3K components disrupts normal glucose uptake and insulin signaling. This target when interacting with proteins like PTEN further influences the development of cancer highlighting its critical role in both oncogenic and metabolic pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).
See full target information PIK3R1 phospho Y467
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 20:20251261 PubMed40918151

2025

CircASH1L-mediated tumor progression in triple-negative breast cancer: PI3K/AKT pathway mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Liu,Jiaming Wu,Jin Gong,Jing Zhu,Jie Liu,Xiaoqing Chen,Shaohua Qu,Jintao Chen,Zhisheng Zhou,Xulong Fan

Annals of translational medicine 10:11 PubMed35242856

2022

CircRASSF2 promotes IGF1R and osteosarcoma metastasis via sponging miR-6838-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyan Wang,Hong Sun,Ke Li,Kun Yang,Yang Xiang,Xiaobin Tian
View all publications
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