Rabbit Recombinant Monoclonal PSGL-1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - APC Anti-PSGL-1 antibody [EPR22504-36] (AB313947), expandable thumbnail](https://content.abcam.com/products/images/apc-psgl-1-antibody-epr22504-36-ab313947--flow-cytometry-img419290.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - APC Anti-PSGL-1 antibody [EPR22504-36] (AB313947)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/12500 | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.(Microbial infection) Acts as a receptor for enterovirus 71.
Alternative names
P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG
Recommended products
Rabbit Recombinant Monoclonal PSGL-1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples.
Alternative names
P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR22504-36
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - APC Anti-PSGL-1 antibody [EPR22504-36] (ab313947), expandable thumbnail](https://content.abcam.com/products/images/apc-psgl-1-antibody-epr22504-36-ab313947--flow-cytometry-img419290.jpg "Flow Cytometry - APC Anti-PSGL-1 antibody [EPR22504-36] (ab313947)")
Flow Cytometry - APC Anti-PSGL-1 antibody [EPR22504-36] (ab313947)
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab313947 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab313947 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 μl at 0.04 μg/ml (1/12500)) for 30 min on ice. The cells were simultaneously stained with CD3.
Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.
Downloads
Product protocols
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