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APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for Flow Cyt (Intra) and reacts with samples. Cited in 1 publication.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

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Reactivity data

Application

Flow Cyt (Intra)

Reactivity

Reacts

Dilution info

-

Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

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APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for Flow Cyt (Intra) and reacts with samples. Cited in 1 publication.

Key facts

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Clone number

EPR25A

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze, Store in the dark

Notes

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

40 product images

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing HeLa cells stained with ab221270 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab221270) (1x106 in 100μL at 0.04, 0.2, 1μg/mL (1/12500, 1/2500, 1/500)) for 30 min at 22°C.

    Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Overlay histogram showing NIH3T3 cells stained with ab223919 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223919, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

    This antibody gave a positive signal in NIH3T3 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Mouse splenocytes cells labelling PSGL-1 with ab322131 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

    Cells are co-stained with CD3 conjugated to Alexa Fluor®488.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Low expression: HEK-293.

    Gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Low expression: LNCaP.

    Gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab322023 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Gated on viable cells.

    Cells are co-stained with CD3 conjugated to Alexa Fluor®488.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Isotype (Left) / Mouse bone marrow treated with 2ug/ml LPS for 24h (Middle) / Untreated mouse bone marrow (Right) cells labelling CD204 with ab320786 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Cells are co-stained with F4/80 conjugated with PE.

    Gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing wild-type Raji (green line) and CXCR5 knockout Raji stained with ab316179 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316179) (1x 106 in 100μl at 0.04 μg/ml (1/12500)) for 30min on ice.

    Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type Raji - black line, CXCR5 knockout Raji - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A20 (mouse reticulum sarcoma B lymphocyte, Left) / RAW 264.7(mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells labelling CD68 with ab317758 at 1/5000 dilution(0.01ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Negative control: A20

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NR8383 (rat alveolar macrophage) cells labelling CD68 with ab317758 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NR8383 (rat alveolar macrophage) cells labelling BTK with ab317755 at 1/5000 dilution(0.01ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling BTK with ab317755 at 1/50000 dilution(0.001ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling BTK with ab317755 at 1/100000 dilution(0.0005ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Negative control: MOLT-4 (PMID:24759210)

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized COS-1 (African green monkey kidney fibroblast-like cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with a CBX1-V5-tagged expression vector containing a myc tag (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling V5 tag with AB317483 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Mouse splenocytes cells labelling CD98 with AB317482 at 1/50 dilution (1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Gated on viable cells.Cells stained with anti-CD4 conjugated to Alexa Fluor® 488.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype contro.

    Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488 and anti-CD45RO (BV421). Gated on viable CD4+ T cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with AB317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488. Gated on viable lymphocytes.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GRP94 with AB317477 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GRP94 with AB317477 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 3h, then together with 300ng/ml BFA for another 3h (Red) / Untreated control (Dotted Red) cells labelling TNF alpha with AB317476 at 1/500 dilution (0.1ug) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50nM PMA and 5ug/ml LPS for 24h (Red) / Untreated control (Dotted Red) cells labelling IL-33 with AB317474 at 1/5000 dilution (0.01ug) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Mouse bone marrow cells cells labelling Sialoadhesin/CD169 with AB317480 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Gated on viable cell. Mouse bone marrow cells are co-stained with F4/80 conjugated PE.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Mouse bone marrow cells cells labelling Sialoadhesin/CD169 with AB317480 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

    Gated on viable cell. Mouse bone marrow cells are co-stained with CD3 conjugated Alexa Fluor®488.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (rat skeletal muscle myoblast) cells labelling Cardiac Troponin T with AB317475 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Cardiac Troponin T with AB317475 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of SH-SY5Y (human neuroblastoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) cells labelling CD98 with AB317481 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cells. Low expression: SH-SY5Y .

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of Wild-type HeLa (human cervix adenocarcinoma epithelial cell, Right) / SLC3A2 (CD98) knockout HeLa (Left) cells labelling CD98 with AB317481 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast) cells labelling CD98 with AB317482 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cells.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD19 with AB317484 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Negative control: Jurkat (PMID: 19147785)

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing wild-type A549 (green line) and GLUT1 knockout A549 stained with ab316298 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316298) (1x 10E6 in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

    Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, GLUT1 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing HeLa cells stained with ab316299 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316299) (1x 10E6 in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

    Isotype control antibody (black line) APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

    This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing left Jurkat positive cells and right negative Hela stained with ab314938 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314938) (1x 106in 100µl at 0.04µg/ml (1/12500)) for 30min at 22°C.

    Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab315146 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab315146) (1x 106in 100µl at 0.2µg/ml (1/2500)) for 30min at 22°C.

    Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315142 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315142 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106in 100 µl at 1.0 µg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with c-Kit.

    Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314955 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314955 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106in 100 µl at 1.0 µg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with CD11b.

    Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.

  • Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814), expandable thumbnail

    Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314954 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314954 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106in 100 µl at 0.2 µg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD56.

    Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable lymphocytes.

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