AB232814

APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb
Recombinant
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Anti-Rabbit IgG, monoclonal [EPR25A] - Isotype Control- APC conjugated (ab232814) is a rabbit recombinant monoclonal antibody used in Flow Cytometry (Intra).

- Biophysical QC for unrivalled batch-batch consistency

40 Images

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cyt

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Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD62L with ab317478 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488. Gated on viable lymphocytes.

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing left THP-1 positive cells and right negative HCT116 stained with ab315146 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab315146) (1x 10⁶in 100μl at 0.2μg/ml (1/2500)) for 30min at 22°C.

Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-10RA with ab325784 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were co-stained with CD14 conjugated to Brilliant Violet 421. Gated on viable cells.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Cells were co-stained with CD4 conjugated to Alexa Fluor®488. Gated on viable lymphocyte.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing left K-562 positive cells and right negative A549 stained with ab314948 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab314948) (1x 10⁶in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Wild-type HeLa (human cervix adenocarcinoma epithelial cell, Right) / SLC3A2 (CD98) knockout HeLa (Left) cells labelling CD98 with ab317481 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / Raji (human Burkitt's lymphoma B lymphocyte, Right) cells labelling CD19 with ab317484 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : Jurkat (PMID : 19147785)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa(human cervical adenocarcinoma epithelial cell) cells labelling beta Tubulin with ab317754 at 1/500 dilution(0.1ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314955 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314955 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶in 100 μl at 1.0 μg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with CD11b.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing left Jurkat positive cells and right negative Hela stained with ab314938 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314938) (1x 10⁶in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Low expression : HEK-293.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CLEC4A with ab322023 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells are co-stained with CD11b conjugated to Brilliant Violet 421.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor®488.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing wild-type A549 (green line) and GLUT1 knockout A549 stained with ab316298 (magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316298) (1x 10E6 in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, GLUT1 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GRP94 with ab317477 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of LNCaP (human prostate carcinoma epithelial cell, Left) / PC-3 (human prostate adenocarcinoma epithelial cell, Right) cells labelling LDL Receptor with ab322128 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Low expression : LNCaP.
Gated on viable cells.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling BTK with ab317755 at 1/100000 dilution(0.0005ug)/Red (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Negative control : MOLT-4 (PMID : 24759210)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing wild-type Jurkat (green line) and IDH2 knockout Jurkat stained with ab317932 (magenta line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab317932) (1x 10⁶ in 100μl at 0.008 μg/ml (1/62500)) for 30min at 22°C.

Isotype control antibody was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control in Jurkat WT cells (black line) and Jurkat-IDH2 KO cells (grey line), used at the same concentration and conditions as the primary antibody.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Cells were co-stained with CD56 conjugated to Brilliant Violet 421. Gated on viable lymphocyte.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Cells were co-stained with anti human CD14 conjugated to Brilliant Violet 421.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Gated on viable cells. Cells were co-stained with anti human CD19 conjugated to PE/CY7.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab314954 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab314954 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice. The cells were simultaneously stained with CD56.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable lymphocytes.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab306538 (right) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10μg/ml human IgG and 10%; normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab306538) or Rabbit IgG (monoclonal) Allophycocyanin (ab232814) isotype (100 μl at 0.04μg/ml (1/12500)) for 30 min on ice. The cells were simultaneously stained with CD19.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on live lymphocytes.

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) THP-1 (human monocytic leukemia monocyte, Right) cells labelling CD300a with ab325381 at 1/500 dilution (0.1ug) / Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells. Negative control : 293T (PMID : 22043923)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and HMGB1 knockout HAP1 stained with ab225041(magenta line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab225041) (1x 10E6 in 100μl at 0.04 μg/ml (1/12500) for 30min at 22°C.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, HMGB1 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / HEK-293T (human embryonic kidney) cells transfected with a human SIGLEC8 expression vector containing a myc-his tag (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling SIGLEC8 with ab318168 at 1/5000 dilution (0.01ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Live cells were surface stained with ab318168, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 488.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) cells labelling CD98 with ab317481 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells. Low expression : SH-SY5Y .

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing wild-type Raji (green line) and CXCR5 knockout Raji stained with ab316179 (red line). The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316179) (1x 10⁶ in 100μl at 0.04 μg/ml (1/12500)) for 30min on ice.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody (wild-type Raji - black line, CXCR5 knockout Raji - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / HEK-293T (human embryonic kidney epithelial cell) transfected with a CBX1-V5-tagged expression vector containing a myc tag (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling V5 tag with ab317483 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing HeLa cells stained with ab316299 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab316299) (1x 10E6 in 100μl at 0.04μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody (black line) APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

This antibody gave a positive signal in HeLa Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a mouse C1QA expression vector containing a Myc tag(Middle) / 293T cells containing a Myc tag (Right) cells labelling 6X His tag with ab317659 at 1/5000 dilution(0.01ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and SMARCA4 knockout HAP1 stained with ab314953 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314953) (1x 10⁶in 100μl at 0.04 μg/ml (1/12500)) for 30min at 22°C.

Isotype control antibody APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, SMARCA4 knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

This antibody gave a positive signal in HAP1 Fixed with 80% methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Cells were stained with anti-CD4 conjugated to Alexa Fluor® 488 and anti-CD45RO (BV421). Gated on viable CD4+ T cells.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Isotype (Left) / Mouse bone marrow treated with 2ug/ml LPS for 24h (Middle) / Untreated mouse bone marrow (Right) cells labelling CD204 with ab320786 at 1/500 dilution (0.1ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells are co-stained with F4/80 conjugated with PE.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of Mouse bone marrow cells cells labelling Sialoadhesin/CD169 with ab317480 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control.

Gated on viable cell. Mouse bone marrow cells are co-stained with F4/80 conjugated PE.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Cardiac Troponin T with ab317475 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Flow Cyt

Lab

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab315142 (right) or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315142 or APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶in 100 μl at 1.0 μg/ml (1/500)) for 30min on ice. The cells were simultaneously stained with c-Kit.

Acquisition of >30000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter. Events were gated on viable cells.

519 FITC

FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control
675 PerCP

PerCP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
578 PE

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
603 Alexa Fluor® 568

Alexa Fluor® 568 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
617 Alexa Fluor® 594

Alexa Fluor® 594 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
519 Alexa Fluor® 488

Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
HRP

HRP Rabbit IgG, monoclonal [EPR25A] - Isotype Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
421 Alexa Fluor® 405

Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
565 Alexa Fluor® 555

Alexa Fluor® 555 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Biotin

Biotin Isotype Control [EPR25A]
775 Alexa Fluor® 750

Alexa Fluor® 750 Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Unconjugated

Rabbit IgG, monoclonal [EPR25A] - Isotype Control
Carrier free

Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free
Carrier free

Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Low endotoxin, Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25A

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, Flow Cyt (Intra), ICC/IF

applications

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.", "FlowCyt-species-checked": "", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab232814) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt).

Other related products
We have a range of other formats of antibody clone [EPR25A] also available for your convenience: ab172730, Alexa Fluor^® 488 - ab199091, Alexa Fluor^® 647 - ab199093, Carrier free - ab199376, HRP - ab199507, Alexa Fluor^® 405 - ab208150, Alexa Fluor^® 594 - ab208568, Alexa Fluor^® 555 - ab208569, PE - ab209478, Alexa Fluor^® 568 - ab209613, Carrier free - ab210849, PerCP - ab222107, FITC - ab223339, APC - ab232814, Biotin - ab320073, Alexa Fluor^® 750 - ab322086

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 16: PubMed39335098

2024

New Advances in the Study of CMTM6, a Focus on Its Novel Non-Canonical Cellular Locations, and Functions beyond Its Role as a PD-L1 Stabilizer.

Applications

Unspecified application

Species

Unspecified reactive species

Pedro Ivan Urciaga-Gutierrez,Ramon Antonio Franco-Topete,Blanca Estela Bastidas-Ramirez,Fabiola Solorzano-Ibarra,Jose Manuel Rojas-Diaz,Nadia Tatiana Garcia-Barrientos,Ksenia Klimov-Kravtchenko,Martha Cecilia Tellez-Bañuelos,Pablo Cesar Ortiz-Lazareno,Oscar Peralta-Zaragoza,Angelica Meneses-Acosta,Alan Guillermo Alejandre-Gonzalez,Miriam Ruth Bueno-Topete,Jesse Haramati,Susana Del Toro-Arreola

eLife 9: PubMed32697192

2020

Host sirtuin 2 as an immunotherapeutic target against tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ashima Bhaskar,Santosh Kumar,Mehak Zahoor Khan,Amit Singh,Ved Prakash Dwivedi,Vinay Kumar Nandicoori

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry (Intracellular) - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

Flow Cytometry - APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB232814)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Product details

Properties and storage information

Form

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Storage buffer

Shipped at conditions