JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB318491

APC Anti-RAS (mutated Q61R) antibody [SP174]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RASN mutated Q61R antibody - conjugated to APC.

View Alternative Names

HRAS1, NRAS, GTPase NRas, Transforming protein N-Ras

  • Carrier free

    Anti-Ras (mutated Q61R) antibody [SP174] - BSA and Azide free

  • Unconjugated

    Anti-Ras (mutated Q61R) antibody [SP174]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Ras (mutated Q61R) antibody [SP174]

  • 578 PE

    PE Anti-RAS (mutated Q61R) antibody [SP174]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-RAS (mutated Q61R) antibody [SP174]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RAS (mutated Q61R) antibody [SP174]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-RAS (mutated Q61R) antibody [SP174]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-RAS (mutated Q61R) antibody [SP174]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP174

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Applications

Antibody Labelling, Target Binding Affinity

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has been found to react with different mutations we tested. We have not tested all possible mutations at Q61.

style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NRAS protein also known as neuroblastoma RAS viral oncogene homolog has a molecular weight of approximately 24 kDa. It functions mechanically as a GTPase cycling between an inactive GDP-bound state and an active GTP-bound state. The NRAS gene which encodes this protein shows expression in various tissues including the thymus leukocytes and other cells of the hematopoietic lineage. Activation of RAS involves the conversion to the GTP-bound form which enables NRAS to transmit signals within cells.
Biological function summary

NRAS plays an important role in cell proliferation differentiation and survival. It does not function alone but is part of the larger RAS protein family including HRAS and KRAS with whom it shares similar roles and sequence homology. NRAS interacts with various growth factor receptors and mediates signals to downstream effectors that influence cellular processes. Mutations in the NRAS gene can affect these biological processes leading to altered cell behavior.

Pathways

NRAS has significant roles in the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways regulate fundamental cellular functions including growth and survival. NRAS interacts closely with other proteins such as RAF kinases and PI3K to propagate signals from activated receptors at the cell membrane to the nucleus. Altered NRAS activity can impact these pathways affecting cellular responses to external stimuli.

NRAS mutations are linked to certain cancers including melanoma and acute myeloid leukemia (AML). Mutant forms of NRAS result in constitutively activated RAS contributing to uncontrolled cell growth and survival. In melanoma NRAS mutations frequently co-occur with alterations in other proteins such as BRAF another member of the RAS/RAF/MEK/ERK pathway. Understanding NRAS's involvement in these conditions is critical for developing targeted therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
See full target information NRAS mutated Q61R
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB313357

Recombinant Human NRAS Protein (His tag) (E.Coli)

Mass Spectrometry - Recombinant Human NRAS Protein (His tag) (E.Coli) (AB313357)

  • 0
  • 0
Cell Lines & Lysates

AB266684

Human NRAS knockout HEK-293T cell line

Advanced Validation

Western blot - Human NRAS knockout HEK-293T cell line (AB266684)

  • 0
  • 0
Cell Lines & Lysates

AB258542

Human NRAS knockout HEK-293T cell lysate

Western blot - Human NRAS knockout HEK-293T cell lysate (AB258542)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com