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Rabbit Recombinant Monoclonal RHOA antibody - conjugated to APC.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regulate cellular responses such as cytoskeletal dynamics, cell migration and cell cycle (PubMed:23871831). Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers (PubMed:31570889, PubMed:8910519, PubMed:9121475). Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis (PubMed:12900402, PubMed:16236794). Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion (PubMed:20974804, PubMed:23940119). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly (PubMed:19934221). The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:19403695, PubMed:9635436). Acts as an allosteric activator of guanine nucleotide exchange factor ECT2 by binding in its activated GTP-bound form to the PH domain of ECT2 which stimulates the release of PH inhibition and promotes the binding of substrate RHOA to the ECT2 catalytic center (PubMed:31888991). May be an activator of PLCE1 (PubMed:16103226). In neurons, involved in the inhibition of the initial spine growth. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity). When activated by DAAM1 may signal centrosome maturation and chromosomal segregation during cell division. May also be involved in contractile ring formation during cytokinesis.(Microbial infection) Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RHOA antibody - conjugated to APC.

Key facts

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18134

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RhoA also known as Ras homolog family member A is a small signaling GTPase. It weighs approximately 21 kDa. This protein is a part of the Rho family which includes other members like RhoB and RhoC. RhoA is widely expressed in various tissues including the brain kidney liver and lungs. Mechanically RhoA functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity regulates the organization of the actin cytoskeleton and influences cell shape attachment and motility.

Biological function summary

The role of RhoA extends to cell proliferation and differentiation. It is a component of the Rho-GTPase cycle interacting with numerous effectors to transmit signals from the cell membrane to the actin cytoskeleton. RhoA influences the assembly of stress fibers and focal adhesions. It plays a part in cellular adhesion and migration making it essential for development and wound healing processes. As a central player in these activities RhoA affects several cellular responses to external stimuli.

Pathways

Research places RhoA prominently in the regulation of the Rho-ROCK (Rho-associated protein kinase) pathway and the planar cell polarity pathway. The Rho-ROCK pathway contributes to cytoskeletal dynamics and cell contractility while the planar cell polarity pathway influences tissue architecture during embryonic development. RhoA interacts with G17A in these pathways coordinating activities such as smooth muscle contraction and neuronal growth. These interactions also involve cross-talk with other signals linked to GTPases like Rac1 and Cdc42.

Associated diseases and disorders

RhoA has connections to cancer progression and hypertension. In cancer RhoA regulates actin dynamics influencing tumor cell migration and invasion. It forms interactions with proteins such as p53 affecting apoptotic pathways and tumor suppression. In hypertension RhoA's role in smooth muscle contraction affects blood vessel tension and blood pressure regulation. Abnormal activity in the Rho-ROCK pathway contributes to these disorders highlighting the significance of RhoA in disease mechanisms.

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