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Rabbit Recombinant Monoclonal SF3B1 antibody - conjugated to APC.

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Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Application

Antibody Labelling

Reactivity

Expected

Dilution info

-

Notes

-

Application

Target Binding Affinity

Reactivity

Expected

Dilution info

-

Notes

-

Target data

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex (PubMed:27720643). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

Alternative names

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Rabbit Recombinant Monoclonal SF3B1 antibody - conjugated to APC.

Alternative names

Key facts

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11986

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.

Supplementary info

Biological function summary

SF3B1 is an essential component of the spliceosomal complex specifically the U2 snRNP component. It helps establish the branchpoint recognition complex ensuring accurate RNA splicing necessary for producing functional mRNA molecules. This process is important for the expression of diverse protein-coding genes affecting multiple aspects of cellular function and identity. The protein interacts with other spliceosomal proteins like SF3A and U2AF to guide the precise removal of non-coding sequences enabling proper translation into proteins.

Activity summary

SF3B1 also known as Splicing Factor 3B subunit 1 plays an important role in the mRNA splicing process. This protein participates in the splicing machinery as part of the spliceosome contributing to the removal of introns from pre-mRNA. SF3B1 has a molecular weight of approximately 160 kDa. It is ubiquitously expressed yet shows a high level of activity in tissues with rapid cell division such as the bone marrow and lymphatic tissue reflecting its central involvement in gene expression regulation.

Pathways

SF3B1 is involved in the mRNA processing pathway and influences the cell cycle pathway. During mRNA processing SF3B1 aids in assembling the spliceosome facilitating the excision of introns. The protein interacts with components like SF3B2 in coordinating splicing with other RNA processing events. In the cell cycle pathway SF3B1 indirectly affects gene expression and stability influencing cell cycle progression and serving as a regulatory node by modulating the splicing of key regulators active during cell division.

Associated diseases and disorders

Several studies associate SF3B1 mutations with certain types of cancer including myelodysplastic syndrome and chronic lymphocytic leukemia. These mutations often result in aberrant splicing events leading to altered gene expression profiles that can promote tumorigenesis. Within these disease contexts SF3B1 abnormalities can influence interactions with related proteins like SRSF2 contributing to misregulated splicing patterns of genes that drive cancerous transformations and affect patient prognosis.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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    Product protocols

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    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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