JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB318168

APC Anti-SIGLEC8 antibody [EPR24419-56]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal SIGLEC8 antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

SAF2, SIGLEC8, Sialic acid-binding Ig-like lectin 8, Siglec-8, Sialoadhesin family member 2, SAF-2

1 Images
Flow Cytometry (Intracellular) - APC Anti-SIGLEC8 antibody [EPR24419-56] (AB318168)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - APC Anti-SIGLEC8 antibody [EPR24419-56] (AB318168)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / HEK-293T (human embryonic kidney) cells transfected with a human SIGLEC8 expression vector containing a myc-his tag (Middle) / 293T transfected with an empty vector containing a myc-His tag (Right) cells labelling SIGLEC8 with ab318168 at 1/5000 dilution (0.01ug) / Middle and Right (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Allophycocyanin) (ab232814) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Live cells were surface stained with ab318168, then fixed with 2% PFA followed by intracellular staining with Myc-tag conjugated to Alexa Fluor® 488.

  • Carrier free

    Anti-SIGLEC8 antibody [EPR24419-56] - BSA and Azide free

  • Unconjugated

    Anti-SIGLEC8 antibody [EPR24419-56]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24419-56

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Proteins & Peptides

AB162108

Recombinant Human SIGLEC8 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human SIGLEC8 protein (GST tag N-Terminus) (AB162108)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SIGLEC8 also known as Sialic acid-binding Ig-like lectin 8 is a protein that functions primarily as a receptor. It has a molecular mass of approximately 50 kDa. SIGLEC8 is expressed on the surface of certain immune cells specifically eosinophils basophils and to a lesser extent mast cells. This receptor plays an important role in the immune responses especially those involving inflammatory processes due to its ability to recognize and bind sialylated glycan ligands.
Biological function summary

SIGLEC8 serves as an inhibitory receptor on these immune cells which can help regulate their activation and survival. It does not form part of a large protein complex but its action can affect various cellular functions such as cell apoptosis and signaling processes. By binding to its specific glycan ligands SIGLEC8 reduces the lifespan of eosinophils which are often involved in allergic responses and asthma thereby helping to control inflammatory responses in tissues.

Pathways

SIGLEC8 is involved in pathways that regulate the immune system particularly those controlling eosinophil apoptosis and inflammation. It interacts with signaling pathways that include the Src family kinases which are central to signal transduction in immune cells. Additionally it shares functional relationships with other receptors in the SIGLEC family which modulate immune responses through similar mechanisms.

SIGLEC8 is significantly associated with asthma and other allergic diseases. The protein's ability to induce apoptosis in eosinophils makes it an important target for therapies aimed at controlling eosinophil-mediated inflammation in asthma. It is also related to the IL-5 signaling pathway as IL-5 plays a role in the survival and activation of eosinophils. By influencing these pathways SIGLEC8 holds potential as a therapeutic target for reducing symptoms and exacerbations in allergic conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells (PubMed : 10625619, PubMed : 10856141). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed : 10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed : 27357658).
See full target information SIGLEC8
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com