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AB278579

APC Anti-SLP76 (phospho Y128) antibody [SLP76Y128-3F8]

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Rabbit Recombinant Monoclonal SLP76 phospho Y128 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Lymphocyte cytosolic protein 2 phospho Y128.

View Alternative Names

Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2

1 Images
Flow Cytometry - APC Anti-SLP76 (phospho Y128) antibody [SLP76Y128-3F8] (AB278579)
  • Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-SLP76 (phospho Y128) antibody [SLP76Y128-3F8] (AB278579)

Flow cytometric analysis of Ramos cells unstained untreated Ramos cells negative control (blue) or stained untreated (red) or treated cells with pervanadate (green) using ab278579.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SLP76Y128-3F8

Isotype

IgG

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human Lymphocyte cytosolic protein 2 phospho Y128. The exact immunogen used to generate this antibody is proprietary information.

Q13094

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.
Biological function summary

This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.

Pathways

The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.

Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed : 11313406, PubMed : 33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed : 8673706, PubMed : 8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed : 11313406). Plays also an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed : 33436632).
See full target information Lymphocyte cytosolic protein 2 phospho Y128
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