Rabbit Recombinant Monoclonal SMAD1 + SMAD5 + SMAD9 phospho S463 + S465 + S467 antibody - conjugated to APC.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Target Binding Affinity | Reactivity Expected | Dilution info - | Notes - |
Application Antibody Labelling | Reactivity Expected | Dilution info - | Notes - |
Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis (PubMed:12064918, PubMed:16516194). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed:9442019). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed:33510867). Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes (PubMed:28675158). Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis (PubMed:28675158).
SMAD1 phospho S463 + S465, SMAD9 phospho S465 + S467
MADH5, SMAD5, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5
Rabbit Recombinant Monoclonal SMAD1 + SMAD5 + SMAD9 phospho S463 + S465 + S467 antibody - conjugated to APC.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467). Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for the Abcam trial program.
The Smad1 SMAD5 and SMAD9 proteins sometimes known as receptor-regulated SMADs (R-SMADs) play significant roles in cellular signaling. These proteins act as intracellular mediators for the transforming growth factor-beta (TGF-β) superfamily of cytokines particularly the bone morphogenetic proteins (BMPs). These proteins are expressed in various tissues including bone heart lung and brain where they facilitate specific receptor signaling. These SMAD proteins typically have molecular masses ranging from about 50 kDa to 60 kDa which varies depending on specific post-translational modifications such as phosphorylation.
The Smad1 SMAD5 and SMAD9 proteins are transcription factors involved in the regulation of gene expression. Upon activation by BMP receptors these proteins undergo phosphorylation then form complexes with SMAD4 another member of the SMAD family. This heteromeric complex translocates into the nucleus where it influences the expression of genes associated with cell proliferation differentiation and apoptosis. The Smad1 SMAD5 and SMAD9 proteins therefore coordinate diverse cellular processes by modulating transcriptional responses to extracellular signals in developmental and homeostatic contexts.
Smad1 SMAD5 and SMAD9 proteins integrate into the BMP signaling pathway which is fundamental in bone and cartilage development. These proteins interact closely with BMP type I receptors through phosphorylation-mediated mechanisms. Another pathway involving these SMAD proteins is the TGF-β pathway where their activity converges with other signaling molecules like SMAD2 and SMAD3 resulting in complex regulatory outcomes in cellular contexts. The interactions within these pathways help mediate processes like embryogenesis and tissue homeostasis illustrating the interplay of cell signaling and transcriptional regulation.
Smad1 SMAD5 and SMAD9 proteins associate with conditions like pulmonary hypertension and cancer. Dysregulation within the BMP signaling pathway mediated by improper functioning of these R-SMADs links to abnormal vascular remodeling and cancerous cell growth due to altered proliferative signaling. Additionally Smad1 and SMAD5 show connections with proteins such as BMPR2 in the context of pulmonary arterial hypertension further emphasizing their role as mediators of disease-related signaling pathways. Addressing these proteins' malfunction provides potential therapeutic intervention points for complex pathological states.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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