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AB314288

APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

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Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to APC. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images
Flow Cytometry (Intracellular) - APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB314288)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB314288)

Flow cytometry overlay histogram showing HeLa cells stained with ab314288 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab314288) (1x 106 in 100μl at 1.0μg/ml (1/500)) for 30min at 22°C.

Isotype control antibody (black line) was APC Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • Biotin

    Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • HRP

    HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • 578 PE

    PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

  • Unconjugated

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

  • Carrier free

    Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB324503

Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular

SimpleStep

Sandwich ELISA - Human/Mouse alpha 1 Sodium Potassium ATPase (ATP1A1) ELISA Kit - Extracellular (AB324503)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

  • 5
  • 1
Primary Antibodies

AB194719

Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB194719)

  • 5
  • 1
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.
Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).
See full target information ATP1A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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