Mouse Monoclonal TRAC antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRBV7-9.
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
| Flow Cyt | |
|---|---|
| Human | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes or 100 Âμl of whole blood. |
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Constant region of T cell receptor (TR) alpha chain (PubMed:24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed:25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed:23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed:15040585).
TRBC2
TCRA, TRAC, T cell receptor alpha chain constant
Mouse Monoclonal TRAC antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRBV7-9.
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA
Recognizes a monomorphic determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
The unconjugated antibody was purified by protein A (G) affinity chromatography (>95% by SDS-PAGE). After conjugation, the antibody was purified by size exclusion chromatography and adjusted for direct use.
Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
The purified antibody is conjugated with Allophycocyanin (APC) under optimum conditions. No reconstitution is necessary.
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The T-cell receptor (TCR) alpha and TCR beta sometimes referred to as alpha beta TCR or alfa TCR are essential components of the immune response machinery. TCR alpha and beta chains each have a mass that varies between 30-40 kDa depending on the variable region's length. These receptor chains are expressed on the surface of T-cells more specifically on the vast majority of T lymphocytes and play a critical role in antigen recognition. Commercial names like anti-TCR or H57 might refer to antibodies targeting these chains.
TCR alpha and beta chains form a heterodimeric complex with other important proteins like CD3 to transmit signals inside the T-cell upon antigen binding. This complex becomes active when T-cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. The interaction between TCR and MHC-peptide complex initializes T-cell activation and subsequent immune responses being central to adaptive immunity.
TCR alpha and beta are integral to the T-cell receptor signaling pathway. This pathway facilitates the activation of important transcription factors such as NF-kB and AP-1 which drive T-cell proliferation and differentiation. The signaling involves several other proteins such as protein kinase C theta and ZAP-70 which further propagate intracellular signaling cascades essential for a fully functional immune response.
Mutations or malfunctions in TCR alpha and beta chains can associate with autoimmune disorders like rheumatoid arthritis and certain types of immunodeficiency. Rheumatoid arthritis involves aberrant TCR signaling pathways that result in inappropriate immune responses against self-antigens. In some immunodeficiencies defective TCR signaling impairs T-cell development and function potentially involving related proteins like CD3 and ZAP-70 leading to compromised immune defenses.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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