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AB269282

APC Anti-TNF alpha antibody [MAb11]

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(2 Publications)

Mouse Monoclonal TNF alpha antibody - conjugated to APC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNF.

View Alternative Names

TNFA, TNFSF2, TNF, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

1 Images
Flow Cytometry (Intracellular) - APC Anti-TNF alpha antibody [MAb11] (AB269282)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - APC Anti-TNF alpha antibody [MAb11] (AB269282)

Intracellular Flow Cytometry analysis (intracellular staining) of activated human peripheral blood labeling TNF alpha with ab269282.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MAb11

Isotype

IgG1

Light chain type

kappa

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human TNF. The exact immunogen used to generate this antibody is proprietary information.

P01375

Specificity

Recognizes human 17-26 kDa cytokine TNF-alpha.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p>Or 100 μL of whole blood.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The TNF protein, primarily secreted by macrophages, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces cell death in specific tumor cell lines and acts as a potent pyrogen, causing fever directly or by stimulating interleukin-1 secretion. TNF is implicated in cachexia induction and can stimulate cell proliferation and differentiation under certain conditions. It impairs regulatory T-cells (Treg) function in rheumatoid arthritis patients through FOXP3 dephosphorylation, upregulating protein phosphatase 1 (PP1), which dephosphorylates 'Ser-418' of FOXP3, inactivating FOXP3 and leading to defective Treg cells. TNF is a key mediator of cell death in the anticancer effect of BCG-stimulated neutrophils with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. It induces insulin resistance in adipocytes by inhibiting insulin-induced IRS1 tyrosine phosphorylation and glucose uptake. TNF plays a role in angiogenesis by inducing VEGF production with IL1B and IL6. Additionally, the TNF intracellular domain (ICD) form stimulates IL12 production in dendritic cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information TNF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108957 PubMed38333692

2024

EP300 restores the glycolytic activity and anti-tumor function of CD8 cytotoxic T cells in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiu Xia,Xiaoxu Ding,Chao Ji,Dabo Zhou,Xun Sun,Tiancong Liu

Evidence-based complementary and alternative medic 2021:8868527 PubMed33505510

2021

Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-B Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Bingfeng Lin,Zhifeng Li,Jie Su,Yulin Feng
View all publications
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