Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to APC. Mitochondrion marker. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Images
![Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB225341), expandable thumbnail](https://content.abcam.com/products/images/apc-tomm20-antibody-epr15581-54-mitochondrial-marker-ab225341--flow-cytometry-intracellular-img26882.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB225341)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
4 products for Alternative Product
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Recommended products
Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to APC. Mitochondrion marker. Suitable for Flow Cyt (Intra) and reacts with Human samples.
Alternative names
Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOMM20, KIAA0016
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
APC
- Excitation/Emission
Ex: 650nm, Em: 660nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR15581-54
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Do Not Freeze, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341), expandable thumbnail](https://content.abcam.com/products/images/apc-tomm20-antibody-epr15581-54-mitochondrial-marker-ab225341--flow-cytometry-intracellular-img26882.jpg "Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)")
Flow Cytometry (Intracellular) - APC Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab225341)
Overlay histogram showing HeLa cells stained with ab225341 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225341, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Allophycocyanin used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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