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Mouse Monoclonal VCAM1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing VCAM1 protein.

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Images

Flow Cytometry - APC Anti-VCAM1 antibody [STA] (AB103173), expandable thumbnail
  • Flow Cytometry - APC Anti-VCAM1 antibody [STA] (AB103173), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing VCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P19320

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
10 µL for 106 Cells
Notes

or 100μl whole blood

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

2 products for Alternative Version

7 products for Alternative Product

Target data

Function

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).

Alternative names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

Recommended products

Mouse Monoclonal VCAM1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing VCAM1 protein.

Key facts

Isotype
IgG1
Conjugation
APC
Excitation/Emission
Ex: 650nm, Em: 660nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing VCAM1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P19320
Clone number
STA
Purification technique
Size-exclusion chromatography
Concentration
Loading...
Purification notes

Conjugates are made from highly purified antibodies (>95% by SDS-PAGE).

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Associated diseases and disorders

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - APC Anti-VCAM1 antibody [STA] (ab103173), expandable thumbnail

    Flow Cytometry - APC Anti-VCAM1 antibody [STA] (ab103173)

    VCAM1 Flow Cytometry staining using mouse Anti-VCAM1 antibody

    Flow cytometry overlay histogram showing wild-type A549 (green line) and VCAM1 knockout A549 cells (red line, Human VCAM1 knockout A549 cell line ab273758), treated with 10 ng/ml TNF-alpha (Recombinant human TNF alpha protein (Active) ab259410) for 16 h (left) and untreated (right), stained with ab103173. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab103173) (1x106 in 100μl at 0.2μg/ml) for 30 min at 4°C.

    Isotype control antibody mouse IgG1κ Allophycocyanin was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; VCAM knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

  • Flow Cytometry - APC Anti-VCAM1 antibody [STA] (ab103173), expandable thumbnail

    Flow Cytometry - APC Anti-VCAM1 antibody [STA] (ab103173)

    VCAM1 Flow Cytometry staining using mouse Anti-VCAM1 antibody

    Surface staining of HDLM-2 cells with ab103173.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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