AB103173

APC Anti-VCAM1 antibody [STA]

KO Validated
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(3 Publications)

Mouse Monoclonal VCAM1 antibody - conjugated to APC. Suitable for Flow Cyt and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing VCAM1 protein.

View Alternative Names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

2 Images

Flow Cytometry - APC Anti-VCAM1 antibody [STA] (AB103173)

Flow Cyt

Lab

Flow Cytometry - APC Anti-VCAM1 antibody [STA] (AB103173)

Flow cytometry overlay histogram showing wild-type A549 (green line) and VCAM1 knockout A549 cells (red line, ab273758), treated with 10 ng/ml TNF-alpha (ab259410) for 16 h (left) and untreated (right), stained with ab103173. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab103173) (1x10⁶ in 100μl at 0.2μg/ml) for 30 min at 4°C.

Isotype control antibody mouse IgG1κ Allophycocyanin was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; VCAM knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 40 mW Red laser (638nm) and 660/10 bandpass filter.

Flow Cyt

Supplier Data

Flow Cytometry - APC Anti-VCAM1 antibody [STA] (AB103173)

Surface staining of HDLM-2 cells with ab103173.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

STA

Isotype

IgG1

Conjugation

APC

Excitation/Emission

Ex: 650nm, Em: 660nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing VCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P19320

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100μl whole blood.</p><p><a href='/en-us/products/primary-antibodies/apc-mouse-fab2-igg1-kappa-monoclonal-15h6-isotype-control-ab37391'>ab37391</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Conjugates are made from highly purified antibodies (>95% by SDS-PAGE).

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

See full target information VCAM1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 26: PubMed35946444

2022

SDF‑1α/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathways .

Applications

Unspecified application

Species

Unspecified reactive species

Xianbin Yuan,Hongya Wu,Xin Li,Lei Chen,Yanhui Xiao,Zhigang Chen,Gaoqin Liu,Peirong Lu

Evidence-based complementary and alternative medicine : eCAM 2021:4836992 PubMed34853599

2021

Jinwujiangu Capsule Treats Fibroblast-Like Synoviocytes of Rheumatoid Arthritis by Inhibiting Pyroptosis via the NLRP3/CAPSES/GSDMD Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ling,Mao Xiao,Zhao-Wei Huang,Hui Xu,Fang-Qin Huang,Ni-Na Ren,Chang-Ming Chen,Dao Min Lu,Xue-Ming Yao,Li-Na Xiao,Wu-Kai Ma

Journal of immunology (Baltimore, Md. : 1950) 154:1069-77 PubMed7529789

1995

Expression of VCAM-1 (CD106) by a subset of TCR gamma delta-bearing lymphocyte clones. Involvement of a metalloprotease in the specific hydrolytic release of the soluble isoform.

Applications

Unspecified application

Species

Unspecified reactive species

G Leca,S E Mansur,A Bensussan

View all publications

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