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AB10923

Anti-Apc1 (phospho S355) antibody

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(1 Publication)

Rabbit Polyclonal APC1 phospho S355 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANAPC1 phospho S355 aa 350-400 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

TSG24, ANAPC1, Anaphase-promoting complex subunit 1, APC1, Cyclosome subunit 1, Mitotic checkpoint regulator, Testis-specific gene 24 protein

1 Images
Western blot - Anti-Apc1 (phospho S355) antibody (AB10923)
  • WB

Unknown

Western blot - Anti-Apc1 (phospho S355) antibody (AB10923)

Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.

All lanes:

Western blot - Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution

Lane 1:

HeLa S3 whole cell lysate (asynchronous cells) at 30 µg

Lane 2:

HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine) at 30 µg

Lane 3:

HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole) at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG [H&L] at 1/5000 dilution

Predicted band size: 216 kDa

Observed band size: 215 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ANAPC1 phospho S355 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H1A4

Specificity

This antibody is specific for phosphorylated human APC1 protein at the pS355 residue. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from chimpanzee and chicken is expected as the sequence of the immunogen only varies by one amino acid in from these sources (89% homology). Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.

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Reactivity data

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Product details

This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS355 residue.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Apc1 protein also known as Anaphase Promoting Complex subunit 1 is a core component of the Anaphase Promoting Complex/Cyclosome (APC/C). With a molecular mass of approximately 215 kDa Apc1 functions within the cell cycle particularly in regulating progression. It is expressed in the nucleus of eukaryotic cells and plays a significant role in governing the ubiquitination and degradation of cell cycle regulators which helps ensure orderly cell division.
Biological function summary

The Anaphase Promoting Complex subunit Apc1 is vital in executing the degradation of securin and cyclin B during the cell cycle. This activity is integral to the transition from metaphase to anaphase during mitosis. Apc1 participates as a structural core within the APC/C complex—a multi-subunit E3 ubiquitin ligase complex. The APC/C complex controls protein ligation marking them for proteasomal degradation and thereby contributing to accurate mitosis and meiosis.

Pathways

Apc1 operates within the cell cycle and ubiquitin-proteasome system pathways. It plays a pivotal part in regulating the metaphase-anaphase transition and exits from mitosis. The protein associates with key cell cycle proteins such as CDC20 and CDH1 which serve as activators for the APC/C complex modulating its activity across different phases of the cell cycle.

The misregulation of Apc1 associates with cancer and certain chromosomal instability syndromes. Aberrant APC/C activity can result in uncontrolled cell proliferation observed in various cancer types including colorectal cancer wherein Apc1 may connect to other proteins like p53 a critical tumor suppressor mutated in many cancers. Altered Apc1 function and consequent cell cycle errors implicate genetic instability which can underlie chromosomal instability syndromes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information ANAPC1 phospho S355
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 39:2613-2623 PubMed29620222

2018

miR‑29a suppresses IL‑13‑induced cell invasion by inhibiting YY1 in the AKT pathway in lung adenocarcinoma A549 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Shujin He,Renmei Mei,Yurong Kang,Jing Duan,Ran Wei,Chuqi Xiang,Yemeng Wu,Xiangtong Lu,Zhenyu Cai,Lixia Xiong
View all publications
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