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AB126204

Anti-Apc10 antibody

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(1 Publication)

Rabbit Polyclonal APC10 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANAPC10 aa 1-150.

View Alternative Names

APC10, ANAPC10, Anaphase-promoting complex subunit 10, Cyclosome subunit 10

1 Images
Western blot - Anti-Apc10 antibody (AB126204)
  • WB

Unknown

Western blot - Anti-Apc10 antibody (AB126204)

12% SDS PAGE

All lanes:

Western blot - Anti-Apc10 antibody (ab126204) at 1/1000 dilution

All lanes:

MCF7 whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ANAPC10 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UM13

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apc10 also known as APC/C subunit 10 or ANAPC10 plays an essential role in cellular processes. This protein weighs around 21 kDa and is a part of the anaphase-promoting complex/cyclosome (APC/C) a critical regulator of the cell cycle. It is expressed in various tissues and contributes significantly to the function of the APC/C which ensures proper cell division by mediating ubiquitin-dependent proteolysis of cell cycle regulatory proteins.
Biological function summary

The anaphase-promoting complex subunit 10 operates as an integral part of the APC/C complex. This multi-subunit ubiquitin ligase complex controls progression through specific cell cycle phases by targeting key regulatory proteins for degradation. APC/C with the help of Apc10 ensures the transition from metaphase to anaphase and is indispensable for exiting mitosis. Apc10 partners with other subunits coordinating the recognition and ubiquitination of target proteins.

Pathways

Researchers place Apc10 within critical cell cycle regulation pathways and ubiquitin-mediated proteolysis. Through these pathways it directly associates with proteins such as CDC20 and CDH1 which are activator proteins that dictate APC/C specificity. Apc10's role in these pathways highlights its function in maintaining cell cycle integrity and ensuring accurate cellular replication.

Apc10 has connections to cancer and cell division-related abnormalities. Its malfunction or misregulation can lead to uncontrolled cell growth as seen in various cancers where the precision of cell cycle control is compromised. Apc10 interacts with other proteins like c-Myc in the context of oncogenic pathways emphasizing its importance in maintaining cellular homeostasis and preventing tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information ANAPC10
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 5:3667 PubMed24770399

2014

Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Sako,Kazuhiro Suzuki,Michitaka Isoda,Satomi Yoshikai,Chiharu Senoo,Nobushige Nakajo,Munemichi Ohe,Noriyuki Sagata
View all publications
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