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AB154546

Anti-Apc11 antibody

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(3 Publications)

Rabbit Polyclonal APC11 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ANAPC11 aa 1-100.

View Alternative Names

HSPC214, ANAPC11, Anaphase-promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein

1 Images
Western blot - Anti-Apc11 antibody (AB154546)
  • WB

Unknown

Western blot - Anti-Apc11 antibody (AB154546)

15% SDS PAGE

All lanes:

Western blot - Anti-Apc11 antibody (ab154546) at 1/5000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 9 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ANAPC11 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9NYG5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apc11 also known as Anaphase Promoting Complex subunit 11 serves a mechanical role in the regulation of the cell cycle. This protein with a molecular mass of approximately 10 kDa is a critical component of the Anaphase Promoting Complex/Cyclosome (APC/C) a large E3 ubiquitin ligase. The Apc11 subunit is expressed ubiquitously across different tissues reflecting its essential function in cellular division and cycle control.
Biological function summary

The small protein is an integral part of the APC/C facilitating the ubiquitination of specific substrates that target them for proteasomal degradation. This process is important for the transition from metaphase to anaphase during mitosis. As part of the APC/C complex Apc11 works closely with other subunits like Apc2 which together enable ubiquitin chain assembly on target proteins. This post-translational modification triggers degradation mechanisms that ensure proper cell cycle progression.

Pathways

Apc11 holds an important position in the cell cycle pathway and the ubiquitin-proteasome degradation pathway. These pathways are essential for maintaining genomic stability by controlling the degradation of regulatory proteins. Apc11 connects with other cycle-regulating proteins such as Cyclin B and Securin both of which are marked for degradation by the APC/C complex. These interactions highlight Apc11’s role in governing the precise timing of cell division events.

The functioning of Apc11 is relevant mainly to cancer and neurodegenerative diseases. Aberrant APC/C activity involving Apc11 can lead to uncontrolled cell proliferation seen in various cancers. Apc11's connection to proteins involved in neurodegenerative processes like tau and ubiquitin might point to its potential impact in disorders such as Alzheimer’s disease. These associations highlight how important correct regulation of this protein is to cellular and overall organismal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Together with the cullin protein ANAPC2, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 11739784, PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 11739784, PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132). May recruit the E2 ubiquitin-conjugating enzymes to the complex (PubMed : 11739784, PubMed : 18485873).
See full target information ANAPC11
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 55:1232-1246 PubMed37258580

2023

SIRT1 ubiquitination is regulated by opposing activities of APC/C-Cdh1 and AROS during stress-induced premature senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Hyup Lee,Ji-Hye Yang,Ui-Hyun Park,Hanbyeul Choi,Yoo Sung Kim,Bo-Eun Yoon,Hye-Jeong Han,Hyun-Taek Kim,Soo-Jong Um,Eun-Joo Kim

Nature communications 13:7732 PubMed36513638

2022

CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Jie Zhou,Xing-Yue Wang,Yan-Hua Dong,Dong-Hui Wang,Zhe Han,Xiao-Jie Zhang,Qing-Yuan Sun,John Carroll,Cheng-Guang Liang

British journal of pharmacology 178:280-297 PubMed32986862

2020

Identification and characterization of a novel adiponectin receptor agonist adipo anti-inflammation agonist and its anti-inflammatory effects in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qiu,Hongle Wu,Zhekai Hu,Xingwen Wu,Maxwell Tu,Fuchun Fang,Xiaofang Zhu,Yao Liu,Junxiang Lian,Paloma Valverde,Thomas Van Dyke,Bjorn Steffensen,Lily Q Dong,Qisheng Tu,Xuedong Zhou,Jake Chen
View all publications
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Product promise

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