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AB122349

Anti-APC15 antibody

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(1 Publication)

Rabbit Polyclonal APC15 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ANAPC15 aa 1-100.

View Alternative Names

C11orf51, HSPC020, ANAPC15, Anaphase-promoting complex subunit 15, APC15

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-APC15 antibody (AB122349)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-APC15 antibody (AB122349)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab122349 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC15 antibody (AB122349)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APC15 antibody (AB122349)

ab122349, at a 1/450 dilution, staining APC15 in paraffin-embedded Human appendix tissue by immunohistochemistry.

Western blot - Anti-APC15 antibody (AB122349)
  • WB

Unknown

Western blot - Anti-APC15 antibody (AB122349)

Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1 : Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

All lanes:

Western blot - Anti-APC15 antibody (ab122349)

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ANAPC15 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P60006

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

APC15 also known as Anaphase Promoting Complex subunit 15 is an important regulatory component of the anaphase-promoting complex/cyclosome (APC/C). This protein with a molecular mass of around 16 kDa plays an important role in cell cycle regulation. APC15 expression occurs in various tissues where it contributes to the timely progression of the cell cycle by controlling the degradation of specific cell cycle regulators. It functions mechanistically by recognizing and targeting substrates for ubiquitination which marks them for degradation by the proteasome.
Biological function summary

APC15 contributes to the molecular machinery that drives cell cycle transitions. It is part of the larger APC/C complex a multi-subunit E3 ubiquitin ligase complex essential for maintaining the proper sequence of cell cycle events particularly during mitosis. APC15's regulatory activity ensures that cells do not prematurely enter anaphase enabling the correct chromosomal alignment and segregation. It interacts with other complex subunits to coordinate precise ubiquitination of proteins that inhibit chromosome separation until the cellular environment is ready.

Pathways

APC15 plays a significant role in cell cycle pathways particularly the cell cycle G2/M transition and mitotic exit. It works closely with other proteins like CDC20 and CDH1 which serve as co-activators to manage progression through the cell cycle. These interactions help direct the APC/C complex to its specific substrates at various stages ensuring fidelity in chromosome segregation and the prevention of aneuploidy a condition with too many or too few chromosomes.

APC15 has a connection to cancer where dysregulation can lead to abnormal cell division and tumorigenesis. This dysregulation often involves overexpression or malfunctioning of associated proteins such as CDC20 impacting the APC/C complex's ability to perform adequate protein degradation and cell cycle control. Additionally defects in the APC/C machinery which includes APC15 have been implicated in neurodegenerative diseases where aberrant protein accumulation and misregulation disrupt normal cellular function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 21926987). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132). In the complex, plays a role in the release of the mitotic checkpoint complex (MCC) from the APC/C : not required for APC/C activity itself, but promotes the turnover of CDC20 and MCC on the APC/C, thereby participating in the responsiveness of the spindle assembly checkpoint (PubMed : 21926987). Also required for degradation of CDC20 (PubMed : 21926987).
See full target information ANAPC15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1534612 PubMed40260298

2025

WGCNA-ML-MR integration: uncovering immune-related genes in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Lv,Yuhua Zhou,Shengkai Jin,Chaowei Fu,Yang Shen,Bo Liu,Menglu Li,Yuwei Zhang,Ninghan Feng
View all publications
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Product promise

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