AB113364

Anti-Apc4 antibody

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(1 Publication)

Rabbit Polyclonal APC4 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ANAPC4 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

APC4, ANAPC4, Anaphase-promoting complex subunit 4, Cyclosome subunit 4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc4 antibody (AB113364)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc4 antibody (AB113364)

ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc4 antibody (AB113364)

ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human uterus tissue by Immunohistochemistry.

Unknown

Western blot - Anti-Apc4 antibody (AB113364)

Lane 1:

Western blot - Anti-Apc4 antibody (ab113364) at 1 µg/mL

Lane 2:

Western blot - Anti-Apc4 antibody (ab113364) at 2 µg/mL

All lanes:

Mouse liver tissue lysate

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ANAPC4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UJX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 99% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apc4 also known as anaphase-promoting complex subunit 4 is a core component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 92 kDa. It is expressed extensively across various tissues and cells due to its integral role in the regulation of the cell cycle. As part of the APC/C Apc4 plays a mechanical role in mediating ubiquitination and subsequent proteolysis of target cell cycle proteins. This regulation helps ensure proper cell cycle progression particularly the transition from metaphase to anaphase.

Biological function summary

Apc4 is fundamental to maintaining genomic stability by facilitating the APC/C’s role as an E3 ubiquitin ligase. The APC/C is an important multi-subunit complex that governs cell cycle checkpoints by tagging proteins for degradation. Apc4 assists in the recognition and selection of substrates ensuring only specific proteins are degraded. Proper function of the APC/C complex is essential for accurate chromosome segregation and normal mitotic exit.

Pathways

Apc4 functions prominently within the ubiquitin-proteasome pathway and the cell cycle regulation network. Apc4 interacts with key regulatory proteins like Cdc20 and Cdh1 which are necessary for the activation and specificity of the APC/C during different cell cycle phases. Through these interactions it ensures orderly degradation of cyclins and other inhibitors facilitating normal cell cycle progression.

Defects or dysregulation in Apc4-related processes have links to cancers and developmental disorders. In certain cancers aberrant expression or mutation of Apc4 leads to chromosomal instability contributing to tumor progression. Research also suggests its interaction with proteins Cyclin B and Securin which when aberrantly regulated further associates with oncogenic pathways. Understanding Apc4’s involvement in these diseases opens avenues for potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).

See full target information ANAPC4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 5:100324 PubMed39803386

2025

Langerhans Cells Directly Interact with Resident T Cells in the Human Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Oka,Tatsuya Hasegawa,Truelian Lee,Valeria S Oliver-Garcia,Mahsa Mortaja,Marjan Azin,Satoshi Horiba,Sabrina S Smith,Sara Khattab,Kathryn E Trerice,Steven T Chen,Yevgeniy R Semenov,Shadmehr Demehri

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Apc4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc4 antibody (AB113364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc4 antibody (AB113364)

Western blot - Anti-Apc4 antibody (AB113364)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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