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AB72149

Anti-Apc4 antibody

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(10 Publications)

Rabbit Polyclonal APC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ANAPC4.

View Alternative Names

APC4, ANAPC4, Anaphase-promoting complex subunit 4, Cyclosome subunit 4

2 Images
Immunoprecipitation - Anti-Apc4 antibody (AB72149)
  • IP

Unknown

Immunoprecipitation - Anti-Apc4 antibody (AB72149)

Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

All lanes:

Immunoprecipitation - Anti-Apc4 antibody (ab72149)

Predicted band size: 92 kDa

false

Western blot - Anti-Apc4 antibody (AB72149)
  • WB

Unknown

Western blot - Anti-Apc4 antibody (AB72149)

All lanes:

Western blot - Anti-Apc4 antibody (ab72149) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 92 kDa

Observed band size: 45 kDa,92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ANAPC4. The exact immunogen used to generate this antibody is proprietary information.

Q9UJX5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apc4 also known as anaphase-promoting complex subunit 4 is a core component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 92 kDa. It is expressed extensively across various tissues and cells due to its integral role in the regulation of the cell cycle. As part of the APC/C Apc4 plays a mechanical role in mediating ubiquitination and subsequent proteolysis of target cell cycle proteins. This regulation helps ensure proper cell cycle progression particularly the transition from metaphase to anaphase.
Biological function summary

Apc4 is fundamental to maintaining genomic stability by facilitating the APC/C’s role as an E3 ubiquitin ligase. The APC/C is an important multi-subunit complex that governs cell cycle checkpoints by tagging proteins for degradation. Apc4 assists in the recognition and selection of substrates ensuring only specific proteins are degraded. Proper function of the APC/C complex is essential for accurate chromosome segregation and normal mitotic exit.

Pathways

Apc4 functions prominently within the ubiquitin-proteasome pathway and the cell cycle regulation network. Apc4 interacts with key regulatory proteins like Cdc20 and Cdh1 which are necessary for the activation and specificity of the APC/C during different cell cycle phases. Through these interactions it ensures orderly degradation of cyclins and other inhibitors facilitating normal cell cycle progression.

Defects or dysregulation in Apc4-related processes have links to cancers and developmental disorders. In certain cancers aberrant expression or mutation of Apc4 leads to chromosomal instability contributing to tumor progression. Research also suggests its interaction with proteins Cyclin B and Securin which when aberrantly regulated further associates with oncogenic pathways. Understanding Apc4’s involvement in these diseases opens avenues for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information ANAPC4
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 22:423-439 PubMed38324016

2024

Coregulation of NDC80 Complex Subunits Determines the Fidelity of the Spindle-Assembly Checkpoint and Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sehong Kim,Thomas T Y Lau,Man Kit Liao,Hoi Tang Ma,Randy Y C Poon

Cell death and differentiation 30:753-765 PubMed36329234

2022

MARCH5 regulates mitotic apoptosis through MCL1-dependent and independent mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Randy Y C Poon

Cell reports 37:109808 PubMed34644583

2021

Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ho Wai Lau,Hoi Tang Ma,Tsz Kwan Yeung,Man Yee Tam,Danyi Zheng,Siu Ki Chu,Randy Yat Choi Poon

Cell reports 34:108929 PubMed33789095

2021

Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Stanislau Yatskevich,Jessie S Kroonen,Claudio Alfieri,Thomas Tischer,Anna C Howes,Linda Clijsters,Jing Yang,Ziguo Zhang,Kaige Yan,Alfred C O Vertegaal,David Barford

EMBO reports 21:e49831 PubMed32307883

2020

A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Alfieri,Thomas Tischer,David Barford

Nature communications 11:24 PubMed31911655

2020

The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Min Jang,Jenny F Nathans,Haiqing Fu,Christophe E Redon,Lisa M Jenkins,Bhushan L Thakur,Lőrinc S Pongor,Adrian M Baris,Jacob M Gross,Maura J OʹNeill,Fred E Indig,Steven D Cappell,Mirit I Aladjem

Nature communications 10:3863 PubMed31455778

2019

Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.

Applications

Unspecified application

Species

Unspecified reactive species

Suyang Zhang,Thomas Tischer,David Barford

PloS one 10:e0118662 PubMed25723520

2015

LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Masuda,Tatsuyuki Chiyoda,Naoyuki Sugiyama,Aldo Segura-Cabrera,Yasuaki Kabe,Arisa Ueki,Kouji Banno,Koji Banno,Makoto Suematsu,Daisuke Aoki,Yasushi Ishihama,Hideyuki Saya,Shinji Kuninaka

The Journal of biological chemistry 286:13052-9 PubMed21335556

2011

Orderly inactivation of the key checkpoint protein mitotic arrest deficient 2 (MAD2) during mitotic progression.

Applications

WB

Species

Human

Hoi Tang Ma,Randy Y C Poon

Proceedings of the National Academy of Sciences of 106:19527-32 PubMed19887645

2009

Cell cycle deregulation by a poxvirus partial mimic of anaphase-promoting complex subunit 11.

Applications

WB

Species

Human

Min Mo,Stephen B Fleming,Andrew A Mercer
View all publications
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