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AB249488

Anti-Apc6 antibody [EPR11168] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Apc6 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ANAPC6, CDC16, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, CDC16 homolog, Cyclosome subunit 6, APC6, CDC16Hs

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169536 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Apc6 with ab169536 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • WB

Unknown

Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Apc6 antibody [EPR11168] (<a href='/en-us/products/primary-antibodies/apc6-antibody-epr11168-ab169536'>ab169536</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from HepG2 cell lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 71 kDa

false

Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)
  • WB

Unknown

Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)

This data was developed using ab169536, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Apc6 antibody [EPR11168] (<a href='/en-us/products/primary-antibodies/apc6-antibody-epr11168-ab169536'>ab169536</a>) at 1/1000 dilution

Lane 1:

HT-1080 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11168

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab249488 is the carrier-free version of ab169536.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Apc6 also known as Anaphase Promoting Complex subunit 6 is essential in cell cycle regulation. It has a molecular mass of approximately 92 kDa. Apc6 is mainly observed in eukaryotic cells reflecting its significant role across diverse organisms. The protein functions as an important player in the Anaphase Promoting Complex/Cyclosome (APC/C) a critical ubiquitin ligase that targets cell cycle regulators for degradation.
Biological function summary

As part of the APC/C complex Apc6 contributes to the ubiquitination and proteasomal degradation of key proteins therefore controlling the cell cycle's transition phases. This regulation ensures orderly progression from metaphase to anaphase by targeting specific cell cycle proteins such as cyclins and securin. The precise role of Apc6 within this complex highlights its importance in maintaining genomic stability especially during the division process.

Pathways

Apc6's involvement in the ubiquitin-proteasome pathway highlights its regulatory action in cell cycle progression. This protein intricately interplays with others like Cdc20 and Cdh1 which serve as activators of the APC/C at different phases of the cycle. Apc6 also aligns itself with the spindle assembly checkpoint pathway providing a surveillance mechanism to prevent premature separation of chromosomes which is critical for preventing aneuploidy.

The malfunction of Apc6 is associated with various forms of cancer highlighting its pivotal role in cell division. Abnormal Apc6 function can lead to uncontrolled cell proliferation and genomic instability conditions often seen in tumor development. Furthermore alterations in Apc6 may connect to neurodegenerative diseases like Alzheimer's possibly due to its regulatory influence over protein degradation processes in neurons.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information CDC16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

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