Rabbit Recombinant Monoclonal Apc6 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Images
![Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--western-blot-img105587.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)")
![Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--western-blot-img24948.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106496.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66107.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (AB249488)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
Alternative names
ANAPC6, CDC16, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, CDC16 homolog, Cyclosome subunit 6, APC6, CDC16Hs
Recommended products
Rabbit Recombinant Monoclonal Apc6 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11168
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249488 is the carrier-free version of Anti-Apc6 antibody [EPR11168] ab169536.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Apc6 also known as Anaphase Promoting Complex subunit 6 is essential in cell cycle regulation. It has a molecular mass of approximately 92 kDa. Apc6 is mainly observed in eukaryotic cells reflecting its significant role across diverse organisms. The protein functions as an important player in the Anaphase Promoting Complex/Cyclosome (APC/C) a critical ubiquitin ligase that targets cell cycle regulators for degradation.
- Biological function summary
As part of the APC/C complex Apc6 contributes to the ubiquitination and proteasomal degradation of key proteins therefore controlling the cell cycle's transition phases. This regulation ensures orderly progression from metaphase to anaphase by targeting specific cell cycle proteins such as cyclins and securin. The precise role of Apc6 within this complex highlights its importance in maintaining genomic stability especially during the division process.
- Pathways
Apc6's involvement in the ubiquitin-proteasome pathway highlights its regulatory action in cell cycle progression. This protein intricately interplays with others like Cdc20 and Cdh1 which serve as activators of the APC/C at different phases of the cycle. Apc6 also aligns itself with the spindle assembly checkpoint pathway providing a surveillance mechanism to prevent premature separation of chromosomes which is critical for preventing aneuploidy.
- Associated diseases and disorders
The malfunction of Apc6 is associated with various forms of cancer highlighting its pivotal role in cell division. Abnormal Apc6 function can lead to uncontrolled cell proliferation and genomic instability conditions often seen in tumor development. Furthermore alterations in Apc6 may connect to neurodegenerative diseases like Alzheimer's possibly due to its regulatory influence over protein degradation processes in neurons.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--western-blot-img105587.jpg "Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Apc6 antibody [EPR11168] (Anti-Apc6 antibody [EPR11168] ab169536) at 1/1000 dilution
Lane 1: HT-1080 cell lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
![Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--western-blot-img24948.jpg "Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Western blot - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Apc6 antibody [EPR11168] (Anti-Apc6 antibody [EPR11168] ab169536) at 1/1000 dilution
All lanes: immunoprecipitation pellet from HepG2 cell lysate
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 71 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Apc6 with Anti-Apc6 antibody [EPR11168] ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106496.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Apc6 with Anti-Apc6 antibody [EPR11168] ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img66107.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Apc6 with Anti-Apc6 antibody [EPR11168] ab169536 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunocytochemistry-immunofluorescence-img98162.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)
This data was developed using Anti-Apc6 antibody [EPR11168] ab169536, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Apc6 with Anti-Apc6 antibody [EPR11168] ab169536 at 1/100 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488), expandable thumbnail](https://content.abcam.com/products/images/apc6-antibody-epr11168-bsa-and-azide-free-ab249488--immunocytochemistry-immunofluorescence-img98162.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] - BSA and Azide free (ab249488)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com