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Rabbit Recombinant Monoclonal Apc6 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (AB250509), expandable thumbnail
  • Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (AB250509), expandable thumbnail
  • Immunoprecipitation - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (AB250509), expandable thumbnail
  • Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (AB250509), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).

Alternative names

ANAPC6, CDC16, Cell division cycle protein 16 homolog, Anaphase-promoting complex subunit 6, CDC16 homolog, Cyclosome subunit 6, APC6, CDC16Hs

Recommended products

Rabbit Recombinant Monoclonal Apc6 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16889
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250509 is the carrier-free version of Anti-Apc6 antibody [EPR16889] ab181567.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Apc6 also known as Anaphase Promoting Complex subunit 6 is essential in cell cycle regulation. It has a molecular mass of approximately 92 kDa. Apc6 is mainly observed in eukaryotic cells reflecting its significant role across diverse organisms. The protein functions as an important player in the Anaphase Promoting Complex/Cyclosome (APC/C) a critical ubiquitin ligase that targets cell cycle regulators for degradation.

Biological function summary

As part of the APC/C complex Apc6 contributes to the ubiquitination and proteasomal degradation of key proteins therefore controlling the cell cycle's transition phases. This regulation ensures orderly progression from metaphase to anaphase by targeting specific cell cycle proteins such as cyclins and securin. The precise role of Apc6 within this complex highlights its importance in maintaining genomic stability especially during the division process.

Pathways

Apc6's involvement in the ubiquitin-proteasome pathway highlights its regulatory action in cell cycle progression. This protein intricately interplays with others like Cdc20 and Cdh1 which serve as activators of the APC/C at different phases of the cycle. Apc6 also aligns itself with the spindle assembly checkpoint pathway providing a surveillance mechanism to prevent premature separation of chromosomes which is critical for preventing aneuploidy.

Associated diseases and disorders

The malfunction of Apc6 is associated with various forms of cancer highlighting its pivotal role in cell division. Abnormal Apc6 function can lead to uncontrolled cell proliferation and genomic instability conditions often seen in tumor development. Furthermore alterations in Apc6 may connect to neurodegenerative diseases like Alzheimer's possibly due to its regulatory influence over protein degradation processes in neurons.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509), expandable thumbnail

    Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)

    This data was developed using Anti-Apc6 antibody [EPR16889] ab181567, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Apc6 antibody [EPR16889] (Anti-Apc6 antibody [EPR16889] ab181567) at 1/5000 dilution

    Lane 1: L-929 (Mouse connective tissue fibroblast cells) whole cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

    Lane 4: HT1080 (Human fibrosarcoma cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

    Observed band size: 71 kDa

  • Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509), expandable thumbnail

    Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)

    This data was developed using Anti-Apc6 antibody [EPR16889] ab181567, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Apc6 antibody [EPR16889] (Anti-Apc6 antibody [EPR16889] ab181567) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: Mouse liver tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

    Observed band size: 71 kDa

  • Immunoprecipitation - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509), expandable thumbnail

    Immunoprecipitation - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)

    This data was developed using Anti-Apc6 antibody [EPR16889] ab181567, the same antibody clone in a different buffer formulation.APC6 Protein was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with Anti-Apc6 antibody [EPR16889] ab181567 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Apc6 antibody [EPR16889] ab181567 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Apc6 antibody [EPR16889] (Anti-Apc6 antibody [EPR16889] ab181567)

    Predicted band size: 71 kDa

  • Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509), expandable thumbnail

    Western blot - Anti-Apc6 antibody [EPR16889] - BSA and Azide free (ab250509)

    This data was developed using Anti-Apc6 antibody [EPR16889] ab181567, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Apc6 antibody [EPR16889] (Anti-Apc6 antibody [EPR16889] ab181567) at 1/5000 dilution

    Lane 1: C6 (Rat glial tumor cells) whole cell lysates at 10 µg

    Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates at 10 µg

    Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 10 µg

    Lane 4: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa

    Observed band size: 71 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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